FAK Antibody - Anticorpo primario, specific to FAK, Rabbit IgG

Cat. No.: Ab102794

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, See COA

Application

IF/ICC, IHC, WB

Host species

Coniglio

Reactive species

consiglio, Mouse, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human FAK Antibody, Polyclonal, could be used for WB, IHC and so on.

Application

WB: 0.5-2 µg/mL

IHC: 5-20 µg/mL

IF/ICC: 3.25 µg/mL

Protein Function

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Specifications

Product Name

FAK Antibody - Anticorpo primario, specific to FAK, Rabbit IgG

Sinonimi

FADK 1 antibody | FADK antibody | FAK related non kinase polypeptide antibody | FAK1 antibody | FAK1_HUMAN antibody | Focal adhesion kinase 1 antibody | Focal adhesion Kinase antibody | Focal adhesion kinase isoform FAK Del33 antibody | Focal adhesion kin

Specifiche e purezza

Carrier Free, ExactAb™, Azide Free, Validated, See COA

Specie ospite

Coniglio

Specificità

FAK

Immunogeno

Recombinant FAK expressed in E.coli (AA 798-1041)

Controllo positivo

WB: C6, NIH/3T3, K562, HUVEC and MCF7 cell lysates. IHC: Human Liver tissue, Human Thyroid tissue, Human Liver cancer tissue. IF/ICC: HeLa cells.

Coniugazione

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Antigen affinity purified and Protein A purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: FAK Antibody (Ab102794)

FAK Antibody (Ab102794) - Western Blot
All lanes: FAK Antibody (Ab102794) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 125 kDa
Observed band size: 111 kDa
Exposure time: 15.6 s

Western Blot: FAK Antibody (Ab102794)

FAK Antibody (Ab102794) - Western Blot
All lanes: FAK Antibody (Ab102794) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 125 kDa
Observed band size: 111 kDa
Exposure time: 15.6 s

IHC: FAK Antibody (Ab102794)

FAK Antibody (Ab102794) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 20 µg/mL FAK Antibody (Ab102794)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: FAK Antibody (Ab102794)

FAK Antibody (Ab102794) - IHC
DAB staining on IHC
Samples: Human Thyroid Tissue
Primary Ab: 20 µg/mL FAK Antibody (Ab102794)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: FAK Antibody (Ab102794)

FAK Antibody (Ab102794) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 20 µg/mL FAK Antibody (Ab102794)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IF/ICC: FAK Antibody (Ab102794)

FAK Antibody (Ab102794) - IF/ICC

IF analysis of FAK Antibody (Ab102794) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with FAK Antibody (Ab102794) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).

Applicazione

• Applicazione: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.5-2 µg/mL;

• IF/ICC:
  3.25 µg/mL

Obiettivi associati (umani)

PTK2 Tclin Focal adhesion kinase 1 (0 Activities)

Discover Target: PTK2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PTK2 Tclin Focal adhesion kinase 1 (4730 Activities)

Discover Target: PTK2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PTK2 Tclin VHL/Focal adhesion kinase 1 (51 Activities)

Discover Target: PTK2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (FAK Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.