CCDC43 Mouse mAb: FAQs for Research Use

CCDC43 Mouse mAb is a monoclonal antibody raised in mouse and designed to detect the coiled-coil domain-containing protein 43 (CCDC43) in a range of research applications.

Product SKU

Ab349464

Antibody Type

Mouse Monoclonal Antibody (mAb)

Target Protein

CCDC43 (Coiled-Coil Domain Containing Protein 43)

Reference Catalog

absea#KC-42846

Recommended Storage

Store at -20°C; avoid repeated freeze-thaw cycles (textbook-level guidance; confirm with datasheet)

Host Species

Mouse

Overview of CCDC43 and Its Biological Relevance

CCDC43 (Coiled-Coil Domain Containing Protein 43) is a member of the coiled-coil domain-containing protein family. Coiled-coil motifs are structural features found in a wide variety of proteins and are involved in mediating protein–protein interactions, subcellular localization, and assembly of multi-protein complexes. CCDC43 has been implicated in cellular structural organization, though its precise functional roles continue to be characterized in ongoing research.

Understanding CCDC43 expression and localization can provide insights into cell biology, particularly processes related to cytoskeletal organization and intracellular trafficking. Reliable detection reagents such as the CCDC43 Mouse mAb (Ab349464) are essential tools for these investigations.

Product Specifications and Recommended Applications

The CCDC43 Mouse mAb (Ab349464) is produced using standard monoclonal antibody technology with mouse as the host species. Monoclonal antibodies offer high specificity and batch-to-batch consistency, making them preferable for quantitative and reproducible research experiments.

Typical applications for monoclonal antibodies of this class include:

• Western Blot (WB): Detection of CCDC43 protein in cell or tissue lysates under denaturing conditions.
• Immunofluorescence (IF): Visualization of CCDC43 localization in fixed cells or tissue sections.
• Immunoprecipitation (IP): Enrichment of CCDC43-containing protein complexes from lysates.
• Immunohistochemistry (IHC): Detection in formalin-fixed, paraffin-embedded (FFPE) tissue sections.

Note: Researchers should verify validated applications from the official product datasheet for Ab349464, as specific application data are not fully detailed in the current source material.

Storage, Handling, and Stability Guidelines

Proper storage and handling of monoclonal antibodies are critical to maintaining their activity and specificity throughout the duration of a research project. The following general guidelines apply to the CCDC43 Mouse mAb (Ab349464):

• Long-term storage: Store at -20°C in a frost-free freezer. For extended storage beyond 6 months, -80°C is recommended.
• Short-term storage: Aliquots may be stored at 4°C for up to 1 month, depending on formulation buffer.
• Freeze-thaw cycles: Minimize the number of freeze-thaw cycles (ideally fewer than 5) to prevent aggregation and loss of activity. Prepare single-use aliquots upon receipt.
• Sodium azide: If the formulation contains sodium azide as a preservative, avoid use in cell-based assays and take appropriate safety precautions.

Always refer to the product-specific datasheet for confirmed storage temperatures, buffer composition, and shelf life.

Troubleshooting Common Issues

Researchers may encounter challenges when working with the CCDC43 Mouse mAb. The following troubleshooting guidance addresses the most frequently reported issues:

• No signal or weak signal (WB): Verify that the protein is expressed in your cell or tissue model. Check protein loading and transfer efficiency. Optimize primary antibody concentration and incubation time. Ensure the secondary antibody is anti-mouse IgG.
• High background: Increase blocking time or optimize blocking agent (BSA vs. non-fat milk). Increase washing stringency (number or duration of washes). Titrate primary antibody concentration downward.
• Non-specific bands: Confirm sample preparation conditions. Consider running a negative control (CCDC43 knockout or knockdown lysate if available).
• Poor immunofluorescence staining: Optimize fixation and permeabilization protocols. Test different antigen retrieval methods for IHC applications.

Contact Aladdin Scientific technical support for application-specific troubleshooting assistance.

Frequently asked questions

What species does the CCDC43 Mouse mAb (Ab349464) react with?

The species reactivity of Ab349464 should be confirmed on the official product datasheet. As a mouse monoclonal antibody targeting CCDC43, it may show reactivity to human and/or mouse CCDC43, but cross-reactivity with other species should be verified experimentally. Please consult the Aladdin Scientific product page or contact technical support for confirmed reactivity data.

Which applications is the CCDC43 Mouse mAb validated for?

Monoclonal antibodies in this class are commonly used in Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), and Immunohistochemistry (IHC). However, the specific validated applications for Ab349464 must be confirmed from the official datasheet. Aladdin Scientific recommends consulting the product datasheet or contacting technical support for application-specific guidance.

How should the CCDC43 Mouse mAb be stored to maintain optimal activity?

As a general guideline for monoclonal antibodies, Ab349464 should be stored at -20°C for long-term preservation and avoid repeated freeze-thaw cycles by preparing single-use aliquots. Short-term storage at 4°C may be acceptable depending on the formulation buffer. Always refer to the specific datasheet for confirmed storage instructions, buffer composition, and expiry information.

What secondary antibody should be used with the CCDC43 Mouse mAb?

Since Ab349464 is a mouse monoclonal antibody, an anti-mouse IgG secondary antibody conjugated to the appropriate detection label (e.g., HRP for Western Blot or a fluorophore for immunofluorescence) should be used. Ensure the secondary antibody is validated for use in your specific application and species model to minimize background and non-specific binding.

What is the reference catalog number associated with this product?

The CCDC43 Mouse mAb (Ab349464) is associated with the reference catalog number absea#KC-42846. Researchers may use this reference number when cross-referencing with supplier databases or when contacting technical support for additional product information.

References

1. Product information for CCDC43 Mouse mAb, Aladdin Scientific, SKU Ab349464, reference absea#KC-42846.
2. Burkhard P, Stetefeld J, Strelkov SV. Coiled coils: a highly versatile protein folding motif. Trends Cell Biol. 2001;11(2):82-88. doi:10.1016/S0962-8924(00)01898-5

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.