KIAA1549L Mouse mAb: Applications, Specificity & Protocol

KIAA1549L Mouse mAb is a mouse-derived monoclonal antibody targeting the KIAA1549L protein, supplied in PBS formulation and intended for use in research applications requiring high specificity against this target.

Product Name

KIAA1549L Mouse mAb

SKU

Ab351269

Host Species

Mouse

Antibody Type

Monoclonal Antibody (mAb)

Formulation

PBS (Phosphate-Buffered Saline) only

Reference Source

Absea catalog number KC-30893

Target Overview: The KIAA1549L Protein

KIAA1549L (also referred to as KIAA1549-like) is a large protein encoded by the KIAA1549L gene. Members of the KIAA gene family were originally identified through large-scale cDNA sequencing projects aimed at cataloguing genes encoding proteins with molecular weights exceeding 50 kDa. The precise molecular function of KIAA1549L remains an active area of investigation; however, it has attracted research interest in part because of its structural similarity to KIAA1549, a gene involved in the KIAA1549–BRAF fusion oncogene commonly detected in pediatric low-grade gliomas.

Understanding the expression pattern and interacting partners of KIAA1549L may provide insight into cell signaling, cytoskeletal organization, or oncogenic processes. Researchers studying brain tumors, developmental biology, or large uncharacterized proteins may find this target relevant to their work.

Product Description & Formulation

The KIAA1549L Mouse mAb (Aladdin SKU: Ab351269) is a mouse monoclonal antibody raised against the KIAA1549L protein. This product is referenced from Absea (catalog number KC-30893) and is formulated exclusively in PBS (phosphate-buffered saline) with no additional carrier proteins or preservatives indicated in the current specification.

Key attributes of monoclonal antibodies in PBS-only format include a defined, homogeneous binding specificity arising from a single B-cell clone, and a carrier-free formulation that offers flexibility for downstream conjugation, labeling, or functional assay development without interference from exogenous proteins such as BSA.

Note: Full technical specifications including isotype, immunogen sequence, lot-specific concentration, and validated applications have not been provided in the current source material. Researchers are advised to contact Aladdin Scientific or consult the product datasheet for complete characterization data before experimental use.

Recommended Handling & Storage Protocol

Proper handling and storage of monoclonal antibodies in PBS formulation are critical to maintaining activity and specificity over time. The following general guidelines apply to PBS-formulated antibodies; always defer to the product datasheet for lot-specific instructions:

• Short-term storage (up to 1 month): Store at 2–8 °C. Avoid repeated freeze-thaw cycles.
• Long-term storage: Aliquot into single-use volumes and store at −20 °C or −80 °C. Adding a carrier protein (e.g., 0.1% BSA) or glycerol (to 50% v/v) may enhance stability if downstream applications permit.
• Handling: Thaw gently on ice or at 4 °C. Mix by brief centrifugation; do not vortex. Use low-protein-binding tubes to minimize adsorption losses.
• Avoid: Repeated freeze-thaw cycles, exposure to temperatures above 25 °C, and prolonged exposure to light if the antibody is conjugated to a fluorophore.

These are standard textbook practices for mAb handling. Confirm specific conditions with the Aladdin Scientific product datasheet.

Potential Research Applications

Monoclonal antibodies targeting specific proteins like KIAA1549L are essential tools for a variety of research applications. While validated application data for Ab351269 should be confirmed via the official datasheet, typical applications for mouse monoclonal antibodies in PBS formulation include:

• Western Blotting (WB): Detection of KIAA1549L protein in cell or tissue lysates under denaturing conditions to confirm expression levels.
• Immunoprecipitation (IP): Enrichment of KIAA1549L and its interacting partners for co-IP or mass spectrometry studies.
• Immunofluorescence (IF) / Immunocytochemistry (ICC): Visualization of KIAA1549L subcellular localization in fixed cells.
• Immunohistochemistry (IHC): Assessment of KIAA1549L expression in formalin-fixed paraffin-embedded (FFPE) or frozen tissue sections.
• ELISA: Quantitative detection in capture or sandwich ELISA formats.

Important: Application suitability must be confirmed with validated data from the product datasheet. Not all applications listed above may have been tested for Ab351269.

Frequently asked questions

What is the formulation of KIAA1549L Mouse mAb (Ab351269)?

The KIAA1549L Mouse mAb is formulated in PBS (phosphate-buffered saline) only, with no carrier proteins or preservatives indicated in the current product specification. Researchers requiring additives such as BSA or glycerol for long-term stability should consult the datasheet or contact Aladdin Scientific.

Which applications has KIAA1549L Mouse mAb been validated for?

Validated application data for Ab351269 is not specified in the current source material. Typical applications for mouse monoclonal antibodies include Western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and ELISA. Please refer to the official product datasheet or contact Aladdin Scientific to confirm which applications have been tested and validated for this specific antibody.

How should KIAA1549L Mouse mAb be stored to maintain activity?

For short-term use (up to one month), store the antibody at 2–8 °C. For long-term storage, aliquot into single-use volumes and freeze at −20 °C or −80 °C to avoid repeated freeze-thaw cycles, which can denature the antibody and reduce its binding activity. Always confirm specific storage conditions with the product datasheet.

What is the relationship between KIAA1549L and KIAA1549 in cancer research?

KIAA1549L shares structural similarity with KIAA1549, which is known for forming the KIAA1549–BRAF gene fusion, a hallmark genomic alteration in pediatric low-grade gliomas. While KIAA1549L itself has not been as extensively characterized, its homology to KIAA1549 makes it a potentially relevant target in cancer biology research. Researchers should consult the current literature for the latest findings on KIAA1549L function.

References

1. Absea Biotechnology. KC-30893 KIAA1549L Mouse Monoclonal Antibody product information.
2. Jones DT, et al. Tandem duplication producing a novel oncogenic KIAA1549:BRAF fusion gene defines the majority of pilocytic astrocytomas. Cancer Res. 2008.
3. Nagase T, et al. Prediction of the coding sequences of unidentified human genes. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 1999.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.