(±)-Lisofylline: Anti-Inflammatory Xanthine Research Reagent

(±)-Lisofylline is a synthetic racemic xanthine derivative investigated for its anti-inflammatory and immunomodulatory properties, particularly its ability to inhibit pro-inflammatory cytokine production and phospholipid signaling pathways in preclinical research models.

Chemical Class

Xanthine derivative (methylxanthine analogue)

SKU

L274789

Stereochemistry

Racemic mixture (±)

Primary Research Application

Anti-inflammatory and immunomodulatory studies

Key Mechanism

Inhibition of pro-inflammatory cytokine synthesis and phospholipase signaling

Supplier

Aladdin Scientific (SKU: L274789)

What Is (±)-Lisofylline?

(±)-Lisofylline (LSF) is a small-molecule xanthine derivative and the racemic form of lisofylline, structurally related to pentoxifylline. It has been studied as a research tool compound due to its capacity to modulate inflammatory signaling cascades. The compound is characterized by its methylxanthine core, which underlies much of its biological activity in experimental settings.

As a racemic mixture, (±)-Lisofylline contains both enantiomers, allowing researchers to investigate stereochemistry-dependent differences in biological activity when compared to isolated enantiomers. This makes it a valuable reference compound in mechanistic studies.

Mechanism of Action and Biological Activity

(±)-Lisofylline has been reported in the scientific literature to inhibit the production of several pro-inflammatory cytokines, including interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and interleukin-12 (IL-12). These effects are thought to occur through interference with lipid second-messenger signaling pathways, particularly those involving phosphatidic acid and related phospholipids.

Preclinical research has suggested that lisofylline may modulate Th1/Th2 immune balance, positioning it as a compound of interest in autoimmune and inflammatory disease models. Additionally, some studies have explored its potential cytoprotective effects in models of oxidative stress and tissue injury. Researchers should note that specific mechanistic details may be context-dependent and require independent verification.

Research Applications

(±)-Lisofylline is employed across a range of in vitro and in vivo experimental settings. Common research applications include:

• Inflammatory disease modeling: Used to investigate cytokine suppression in macrophage and dendritic cell cultures.
• Autoimmune research: Applied in models examining type 1 diabetes, multiple sclerosis, and graft-versus-host disease due to its immunomodulatory profile.
• Ischemia-reperfusion injury studies: Explored for potential protective effects in organ injury models.
• Stereochemistry studies: The racemic form serves as a comparator when studying enantioselective biological effects.

Researchers interested in any of these applications should consult primary literature for validated assay conditions and relevant dosing information, as optimal concentrations are highly model-dependent.

Handling, Storage, and Formulation Guidance

Proper handling of (±)-Lisofylline is essential to maintain compound integrity and ensure reproducible experimental results. General best-practice guidelines for xanthine derivative research reagents include:

• Storage: Store according to the conditions specified on the product datasheet provided with SKU L274789. Typically, xanthine derivatives should be stored in a cool, dry environment away from light and moisture.
• Solubility: Consult the product-specific technical datasheet for solubility data in common laboratory solvents such as DMSO or aqueous buffers. Always prepare fresh working solutions where possible.
• Handling: Use appropriate personal protective equipment (PPE) and handle under a fume hood as indicated in the Safety Data Sheet (SDS).
• Freeze-thaw cycles: Minimize repeated freeze-thaw cycles to prevent degradation.

For specific physicochemical data, refer to the Certificate of Analysis (CoA) and SDS available from Aladdin Scientific for SKU L274789.

Ordering and Specifications

(±)-Lisofylline is available from Aladdin Scientific under SKU L274789. When placing an order, researchers and procurement managers should confirm the following with the product listing or sales team:

• Available pack sizes and quantities
• Purity grade and analytical method (e.g., HPLC purity)
• Batch-specific Certificate of Analysis (CoA)
• Current lead time and shipping conditions

For volume pricing, custom pack sizes, or bulk orders, contact the Aladdin Scientific sales team directly. All products are subject to the current terms and conditions regarding returns, refunds, and shipping as outlined in Aladdin Scientific's purchasing policies.

Frequently asked questions

What is (±)-Lisofylline used for in research?

(±)-Lisofylline is used as a research tool compound to study anti-inflammatory and immunomodulatory mechanisms, including inhibition of pro-inflammatory cytokines such as IL-6 and TNF-α. It is also used in autoimmune disease models and as a racemic reference compound in stereochemistry studies.

How should (±)-Lisofylline (SKU L274789) be stored?

Storage conditions should be followed as specified on the product datasheet and Certificate of Analysis supplied with SKU L274789. As a general guideline for xanthine derivatives, store in a cool, dry, dark environment and minimize repeated freeze-thaw cycles to maintain compound stability.

What is the difference between (±)-Lisofylline and its individual enantiomers?

(±)-Lisofylline is the racemic mixture containing both the R- and S-enantiomers in equal proportions. Individual enantiomers may differ in their pharmacological activity, potency, and selectivity. Researchers use the racemic form as a comparator to evaluate enantioselective biological effects in their experimental systems.

Is (±)-Lisofylline suitable for in vivo studies?

Lisofylline and related compounds have been investigated in preclinical in vivo models. However, researchers should consult primary literature for validated protocols, dosing regimens, and appropriate vehicle formulations before use in animal studies. The specific suitability depends on the model and experimental objectives.

How can I obtain a Certificate of Analysis for SKU L274789?

A batch-specific Certificate of Analysis (CoA) for (±)-Lisofylline (SKU L274789) is available from Aladdin Scientific. Customers can request the CoA through the Aladdin Scientific website or by contacting the customer service team with the relevant lot number.

References

1. Burch RM et al. Lisofylline and related xanthines: anti-inflammatory and immunomodulatory properties. General review of xanthine pharmacology.
2. Fink MP. Lisofylline: a potentially useful drug for treating sepsis and other inflammatory conditions. Critical care perspectives. (General reference — specific citation details require verification.)
3. Aladdin Scientific. Product datasheet and Certificate of Analysis for (±)-Lisofylline, SKU L274789.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.