Confined transduction experiments with λ phage
Lambda phage is a kind of mild phage. In the host cell of E. coli, its DNA can be integrated in the host chromosome and passed on to the offspring cells just li
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Restriction endonuclease profiling of λ phage DNA
Lambda phage is a commonly used biological material in molecular genetics research, and the enzymatically fragmented segments of its DNA are often used as stand
Non-isotopic labeling of DNA probes
DNA probes are divided into two categories: isotope-labeled probes and non-isotope-labeled probes. Isotope-labeled probes usually have high radioactivity and hi
Isolation and purification of chloroplast DNA from plant cells
Chloroplasts are unique organelles in plant cells and contain DNA. chloroplast DNA is in the form of a ring, and the chloroplast DNA (cpDNA) of higher plants va
Gene Complementation Experiment
Genes are the smallest functional units of biological genetic material, and genes are separable. To determine the boundaries of a gene, it is not possible to re
Deletion localization-gene fine structure analysis
Deletion localization is a gene localization method in which a strain at the mutation position to be tested is recombined separately with a series of mutant str
Concurrent transduction and gene localization--a three-dot hybridization experiment
The distance between two genes can be measured by the number of recombinants in the progeny after two- and three-point crosses. The closer the distance between
Extraction of mitochondrial DNA from plant cells
In eukaryotes, in addition to the nuclear genome, there are cytoplasmic genomes, i.e., the mitochondrial genome and, in green plants, the chloroplast genome. Bo
Bacterial splicing and gene localization-disrupted hybridization
It is a method of range localization by physically interrupting the binding action of bacteria.