Experiments on the preparation of inhomogeneous ribonucleoprotein complexes
In the nucleus, the transcription products of RNA polymerase II bind to a large number of different types of nuclear precursor mRNA/mRNA-binding proteins, heter
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Experimental detection of protein-double-stranded RNA interactions by methylene blue-mediated photocrosslinking reaction
Methylene blue-mediated photocrosslinking reactions are a complement to UV crosslinking reactions. Methylene blue belongs to the phenothiazine group of dyes. Du
deoxyribonuclease assay
With the development of in vitro molecular experimental techniques, many DNA molecules with different catalytic functions have been discovered in recent years.
In vivo isolation of ribonucleoprotein complex by immunoprecipitation-rPCR of RNA
The RNA Immunoprecipitation-Randomized Polymerization Chain Reaction method can be used to identify RNA components in intracellular RNP complexes. This experime
Oligodeoxythymidine cellulose purification band poly( A)+RNA
Most mammalian messenger RNAs (mRNAs) carry a poly(A)+ tail, and Poly(A)+-carrying RNAs can be isolated from total RNA using this protocol.
Biotinylated oligodeoxythymidine-streptavidin magnetic bead purification band Poly(A)+RNA
The use of magnetic microspheres instead of transferring fiber cords as a medium is more commonly used and has been commercialized.
Experimental purification and removal of nucleoprotein complexes by antisense affinity chromatography
This experiment was derived from "RNA Laboratory Guidebook", edited by Xiaofei Zheng.
Solubilization and preservation of RNA in formamide
Storing RNA in formamide has many advantages over storing it in water or ethanol: (1) formamide will protect the RNA from degradation by RNA enzymes, and RNA st
Chromosome preparation experiments on cultured peripheral blood cells
Although chromosomes can be prepared from other cells, human peripheral blood leukocytes are the easiest to synchronize, allowing high-resolution analysis of th