T4 DNA ligase ligates RNA molecules
T4 DNA Ligase joins double-stranded complexes, including RNA-DNA heteroduplexes and RNA-RNA heteroduplexes, defectively.
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Deoxyribonuclease cleaves RNA at a specific site
Deoxyribonucleases are oligodeoxyribonucleotides consisting of about three dozen deoxyribonucleotides, which are easy to prepare and convenient to use, and are
RNA reverse transcription
The RNA template is used to obtain DNA by reverse transcription, which is then used to replicate and infect cells.
Nuclease cleaves RNA at a specific site
Nuclease is a useful tool for obtaining site-specific cleavage products of long fragments of RNA for the study of RNA modification, for modification site analys
A simple and effective transcription method to reduce heterogeneity at the 3' end of RNA
Ribosyl C2'-methoxy modification of the nucleotides at the 2 5' ends. Amplification of DNA by PCR with modified primers still reduces N+l activity, and since PC
In vitro translation system for prokaryotic cells
The S30 family of extracts is divided into three categories: the E. coli S30 Extract System for in vitro translation of circular DNA, the E. coli S30 Extract Sy
An in vitro translation system for African Xenopus oocytes
The African Xenopus oocyte in vitro translation system is capable of sustained modification and processing of most exogenous mRNAs due to its ability to translo
mRNA 3' end processing in vitro
There are two strategies for in vitro experiments; one is to co-incubate a plasmid containing the target gene at the Po ly( A) locus with cell-free extracts to
Translation of mRNA in wheat germ extracts
Wheat germ extract in vitro translation system for efficient translation of mRNA in viruses and eukaryotic cells in a heterogeneous cell-free system