Northwestern screening of protein expression library assay
Northwestern screening of protein expression libraries is performed by constructing IPTG-inducible fusion protein expression libraries (the N-terminus of the fu
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RNaseH cleavage protection experiments for oligonucleotide targeting in RNA- protein complexes
Oligonucleotide-targeted RNase II digestion assay is a method to analyze RNA protein complexes (RNP, ribonucleoprotein particles), both for crude extracts and p
Large-scale preparation of Drosophila RNA
This method allows for the preparation of RNA from 100 Drosophila or approximately 2g of Drosophila embryos.
Analysis of RNA-peptide interactions by PACE
The polyacrylamide coelectrophoresis (PACE ) assay is a relatively recently developed method for quantitatively analyzing the interaction of proteins and peptid
Yeast three-hybrid system for detecting and analyzing RNA-protein interactions
The yeast three-hybrid system can be used to analyze protein-RNA interactions. In the yeast three-hybrid system, RNA-protein interactions lead to transcription
Total RNA or poIy(A) +RNA from Drosophila embryos
RNA Laboratory Techniques Manual (Molecular Cloning - Laboratory Guide Series)
Determination of RNA-protein dissociation constants by nitrocellulose filter membrane binding assays
The binding of proteins to DNA and RNA can be determined using nitrocellulose membrane binding, which is based on the fact that most proteins bind to nitrocellu
Liquid-phase screening of RNA-binding proteins in cDNA expression libraries
The method of immobilizing cDNA library-expressed proteins on nitrocellulose or nylon membranes and then screening them with labeled RNA is the most commonly us
Extraction and purification of RNA from polysaccharide-rich plant tissues
RNA Laboratory Techniques Manual (Molecular Cloning - Laboratory Guide Series)