Rapid isolation of yeast DNA
Yeast DNA prepared according to this protocol can be used as a template for PCR reactions. The shuttle plasmid, which replicates in both E. coli and Saccharomyc
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Isolation of DNA from mammalian cells grown in 96-well microculture plates
This protocol, modified from the method of Ramirez-solis et al. 1992, 1993, is a simple and efficient method for extracting genomic DNA from eukaryotic cells gr
Isolation of DNA from mammalian cells by the winding method
This method is a modification of the Bowtell method (1987), which allows for the simultaneous preparation of DNA from many different cell or tissue samples.The
Growth of Saccharomyces cerevisiae and its DNA preparation
DNA prepared by this method is suitable for agarose gel electrophoresis, Southern blotting, subcloning, genomic library construction, PCR, or other methods that
Isolation of high molecular mass DNA from mammalian cells using formamide
This protocol is a modified version of the method described by Kupiec et al. (1987), which consists of digestion of cells and tissues with proteinase K, separat
Southern blotting (capillary transfer of DNA to a membrane)
Genomic DNA samples are prepared by digestion with one or more restriction endonucleases, and the digested fragments are separated by electrophoresis on a stand
Recovery of DNA from low melting point agarose gels (organic solvent extraction)
Hydroxyethyl-modified agarose reduces the number of hydrogen bonds between chains and can melt and solidify at lower temperatures than standard agarose. The ext
Purification of DNA recovered from agarose and polyacrylamide gels by anion-exchange chromatography
DNA fragments recovered from agarose gels, such as PCR products or digested DNA fragments, are resistant to further digestion. The reason for this resistance is
Transfer and immobilization of denatured RNA on membranes
In most cases, to detect a specific target mRNA, the RNA is separated by agarose electrophoresis and then transferred from the gel to a two-dimensional support,