Isolation experiments of single nucleated cells in mice
The basic techniques for preparing cell suspensions from mouse lymphoid organs are first described, including the removal of erythrocytes and dead cells The met
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3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Preparation of antibodies recognizing specific tyrosine phosphorylated polypeptides
Antibodies against phosphopeptides recognize proteins that are only in the phosphorylated state. The antibody does not cross-react with unphosphorylated protein
Separation and application of lipid rafts
Lipid rafts are important in immunology because T-cell receptors and several other important T-cell signaling proteins are present in lipid rafts or are recruit
T lymphocyte proliferation assay
This module introduces the mouse T lymphocyte proliferation assay. Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from the "Comp
Detection of apoptosis and other forms of cell death
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Phenotyping of Human Monocytes/Macrophages
To provide a phenotypic assay for human monocytes/macrophages. Author: J.E. Colligan et al, Translator: Xuitao Cao et al. This experiment is from the "Comprehen
Detection of intracellular factors by flow cytometry
This unit describes the use of 4-color fluorescence to analyze the levels of IFN-7, IL-4 and IL-2 expressed in CD4+ T cells. (can be used to calculate T h l and
Immunofluorescence and cell separation
Flow cytometry is used to analyze membrane surface antigens and intracellular antigens expressed by cells, and is capable of specifically distinguishing differe
Monoclonal antibody ascites preparation experiment
The primary advantage of using monoclonal antibodies (MAb) over polyclonal antisera is the possibility of obtaining large quantities of specific monoclonal anti