Cold Trypsin Dissociation of Tissues
The cut tissues were placed in trypsin at 4°C for 16-18 h. After removing the trypsin, the cells were warmed and dispersed in warm medium.
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Experiments for the determination of growth fraction
Cells were labeled continuously for 48h and sampled at intervals for radioautography. Source: Animal Cell Culture: A Guide to Basic Techniques, Fifth Edition
Radiation autoradiography in in situ hybridization
Isotope-labeled probes were hybridized with cells fixed on microscope slides, and then the hybridization sites were visualized by radiographic autoradiography.
time-lapse camera
Cells are cultured in culture chambers with suitable optical microscope characteristics. The microscope is fitted with a camera connected to a gas recorder, an
Fluorescence in situ hybridization using single-copy genomic probes and chromosome coloring methods
Hybridization of biotin-labeled or digoxigenin-labeled probes to chromosomes detects degenerate chromosomes, and individual probes can be detected by double-ant
Stimulation of lymphocytes with PHA
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Real-Time Label-Free Dynamic Cell Analysis
RTCA real-time detection of live cell activity
Expansion of insect cells
Cells were mechanically isolated from the cell monolayer and cultured for expansion at 27°C and in suspension.
Liposome-mediated transient transfection
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)