Construction of multiple cis-trans vectors linked by 2A polypeptides
This chapter describes methods for expressing multiple proteins from a single readable frame by using 2A polypeptide-linked polycis-trans vectors. When these sm
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Simian vacuolar virus type 1 vector
Vacuolar viruses (FV) are non-pathogenic retroviruses that efficiently and safely transfer genes to different types of cells in different species. These viruses
Small RNA virus-based expression vectors
Small R N A viruses comprise a large group of small, pod-less, positive-sense single-stranded R N A viruses. Small R N A viruses have a rapid life cycle that ta
Polyomavirus: SV40
This chapter describes how to construct H S V /A A V heterotrimeric vectors using an unassisted viral packaging system, and also discusses its advantages and di
Screening, isolation and characterization of target peptides for ligand-directed gene delivery
Functional vascular mapping of tissue-specific and angiogenesis-related receptors has been more widely promoted and optimized by isolation of receptors, cell li
Delivery of genes with HSV/AAV heterozygous amplicon vectors
This chapter describes how to construct H S V /A A V heterotrimeric vectors using an unassisted viral packaging system, and also discusses its advantages and di
Stable virus-producing cell lines for AAV assembly
Cloning of virus-producing cells by infection with a helper virus, stable virus-producing cell lines containing and genes, and r A A V (recombinant adeno-associ
In vitro packaging of SV40: a pseudoviral gene delivery system
Nuclear extracts from baculovirus-infected insect cells of the meadow moth (Sf9 ) encode the major capsid protein (V P l ) of simian virus 40 (S V 4 0 ). In the
Production and characterization of helper virus-dependent adenoviral vectors
This chapter describes in detail the preparation, amplification and large-scale production of helper virus-dependent adenoviral vectors (H D A d ) for use in ge