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Laboratory Protocols & SOPs Library

3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.

3228 articles
Laboratory Protocols & SOPs Library

Terrific Broth, Modified for Fermentation, non-animal (Powder)

Terrific Broth is a highly enriched bacteriological medium specifically designed by Tartof and Hobbs to increase the yield of plasmid DNA derived from suspension cultures of E. coli due in part to the increased buffering capacity derived from using mono and dibasic phosphates.

2025-06-10 03:33:44 Updated
Laboratory Protocols & SOPs Library

Terrific Broth, Modified for Genomics (Powder)

Terrific Broth is a highly enriched bacteriological medium specifically designed by Tartof and Hobbs to increase the yield of plasmid DNA derived from suspension cultures of E. coli due in part to the increased buffering capacity derived from using mono and dibasic phosphates.

2025-06-10 03:33:44 Updated
Laboratory Protocols & SOPs Library

Two YT Broth (TY Broth) (Powder)

Media for the propagation of M13 and filamentous bacteriophages in E. coli K12 strains, according to Miller

2025-06-10 03:33:44 Updated
Laboratory Protocols & SOPs Library

YT Broth (Powder)

Media base for propagation of bacteriophage lambda.

2025-06-10 03:33:44 Updated
Laboratory Protocols & SOPs Library

B5 Fixative Recipe

B5 Fixative Recipe

2025-06-10 03:33:44 Updated
Laboratory Protocols & SOPs Library

Staining Dead Cells with Viability Dyes

The Viability Dyes can be used to exclude non-viable cells in flow cytometry assays. They irreversibly bind to cell su rface and intracellular amines. The dye can covalently bind to a higher concentration of amines in cells that have lost plasma membrane integrity, such as those in the late ...

2025-06-10 03:33:44 Updated
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