Experiments on nanogene vectors based on polylactic acid and polyethylene glycol
Nanoparticles combining polylactic acid-hydroxyglycolic acid (PLGA) and polyethylene glycol (PEG) derivatives have been used as gene carriers for mucosal permea
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3228 articles
Transposon-mediated delivery of small interfering RNAs: the "Sleeping Beauty" transposon experiment
A method for efficient delivery of pol III-driven SHRNA expression frames using the Sleeping Beauty (SB) transposon. Advantages of this system include its ease
Cross-linked hyaluronic acid matrix and film experiments with specific plasmid DNAs
Natural polysaccharides are being extensively studied as carriers for gene delivery. The non-inflammatory and non-immunogenic nature of hyaluronic acid (H A ) i
Expression and characterization of ribozymes and short hairpin RNA in mammalian cells
Functional genomic research using R N A interference techniques (R N A i ) has been carried out extensively in recent years.R N A i is the newest and most succe
Experiments on a highly sensitive gene regulatory system based on a synthetic small-molecule ligand-conducted decidual hormone receptor
The RheoSwitch system is an efficient gene regulatory system based on the ecdysone receptor, which is activated by synthetic small molecule ligands. The recepto
Genetic experiments for manipulating mammalian cells by microinjection
Introduction by microinjection can be used for any type of adherent cell, including primary cells. Since siRNA for any target gene can be obtained simply and ra
Sonopuncture: an effective technique for introducing genes into chicken embryos
The acoustic pore effect may not be applicable to the introduction of genes into tissues with lumen-forming or open structures. Acoustic pore effects on neural
Reassembly experiments of human artificial chromosomes based on bacterial artificial chromosomes
Successful gene transfer requires efficient transport and long-term presence of exogenous genes in order to maintain physiologically relevant and appropriately
Efficient DNA delivery by phage into mammalian cells
Transferring genes into cells and expressing new proteins, or in this way altering the expression levels of endogenous proteins, can modulate the physiological