Preparation of electroporation-transformed receptor E.coli TG1 cells
This experiment was mainly used to prepare receptorized E.coli TG1 cells.
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3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Expression profiling gene chip experiments
Expression profiling gene chips can be applied to (1) disease diagnosis; (2) new drug development; and (3) environmental protection.
Experiments in searching bioinformatics databases and software
Database is the main content of bioinformatics, and various databases cover almost all fields of life sciences; the main functions of bioinformatics software ar
Preparation of blood samples for gene chip analysis
Preparation of blood samples for gene chip analysis can be used for (1) gene expression analysis and (2) analysis of gene expression in blood leukocytes.
Cell proliferation assay: MTT method
The MTT assay, also known as the MTT colorimetric assay, can be used to: detect cell survival and growth.
Transfection of HEK293T cells by calcium phosphate method
Transfection of HEK293T cells by calcium phosphate method can be applied to (1) study the mechanism of transfection of mammalian cells and (2) genetic engineeri
Determination of chloroplast periplasmic integrity
Determination of chloroplast periplasmic membrane integrity can be used for (1) studies of the molecular mechanisms that promote photosynthesis and (2) detectio
Live staining and dead-viable identification of plant cells
In vivo staining can be used to (1) show certain natural structures within the cell using certain non-toxic or minimally toxic dyes, and (2) not affect the life
Gene annotation prokka
Prokaryotic genome assembly and sequencing has a mature methodology, but there is no fast and easy way to annotate the assembled genome. Prokka: rapid prokaryot