Chlorophyll saponification assay
Chlorophyll is an ester of a dicarboxylic acid that saponifies with a base to form a basic salt of chlorophyllic acid; after the formation of the salt, chloroph
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3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Experiments for the observation of protoplasmic movement
Within living cells, the phenomenon of active movement of protoplasm is collectively known as protoplasmic movement. Protoplasmic movement is one of the importa
Catalase activity assay experiment
H2O2 is strongly absorbed at 240 nm, and catalase decomposes hydrogen peroxide, causing the absorbance of the reaction solution (A240) to decrease with reaction
Experimental morphological observations on vesicles in young wheat roots
The tip of the germinating wheat embryonic root was cut (about 1 cm from the tip) and immersed in neutral red solution for live staining for 10-15 min, then rem
Experiments on the separation of liquid bubbles
Tear the epidermis of purple onion or radish leaves, put it on a slide, add a little drop of water and then cover the coverslip, observe the cells in the prepar
Experiments on the determination of photosynthetic intensity of plants
According to the similarity of the structure and physiological function of the two sides of the leaf midvein, you can first cut off half of the leaf in the dark
Plant respiration intensity measurement experiment
The intensity of respiration can be expressed as the number of CO2mg released per unit weight of plant material per unit time. The CO2 released by respiration i
Experiments on the substitution of hydrogen and copper for magnesium in chlorophyll
Chlorophyll molecule has a porphyrin ring, its magnesium ions are located in the center of the porphyrin ring, chlorophyll molecule is more unstable, in contact
Experiments on oxidation and phosphorylation in isolated mitochondria
When sufficient oxygen is supplied to plant tissues, plant cells can oxidize substrates completely. When glucose is fully oxidized as a respiratory substrate, C