Laboratory Protocols & SOPs Library
- Concentration or potency assay of cytokinin-like substances by the radish cotyledon weight gain method SummaryCytokinins have the effect of promoting cotyledon enlargement in radish. Over a range of concentrations (0.05 to 50 pp M for 6-BA agonist), cotyledon weight gain was linearly related to the concentration ofOperation methodConcentration or potency assay of cytokinin-like substances by the ...
- Experiments on the growth of pollen tubes and their orientability SummaryMature pollen falling on the stigma will germinate and grow pollen tubes, and pollen germination can also occur when appropriate conditions (temperature, pH, osmotic pressure of the medium) are given artificially. Pollen germination and the growth of pollen tubes require a certain amount ...
- Experiments on the effects of the ripening action of ethylene SummaryEthylene is one of the five major endogenous hormones and the only gaseous hormone. Almost all plant tissues have the potential to produce ethylene. Ethylene has a variety of physiological functions in plants, the most important of which include: promotion of fruit ripening, "triple ...
- The "triple reaction" experiment with yellowing pea seedlings SummaryEthylene is a gaseous hormone that has multiple effects on plant metabolism, growth and development. Treatment of yellowing pea seedlings with ethylene gas inhibited the elongation of the seedling epigyne, and caused the epigyne to expand and grow laterally. These three responses of ...
- Ethylene-induced female flower formation experiment SummaryEthylene alters membrane permeability and cell compartmentalization, and induces the synthesis, release or activation of many enzymes (especially degradative enzymes), which is one of the main reasons why ethylene promotes maturation and abscission. The synthesis of new RNAs and proteins ...
- Observational experiments on Hill's reaction SummaryHill reaction (Hill reaction) is a reaction in which isolated chloroplasts of green plants decompose water in the presence of light, releasing oxygen and at the same time reducing the electron acceptor. The oxidant 2,6-dichlorophenol indophenol is a blue dye that accepts electrons and H+ ...
- Experiments for the determination of the total water content of plant tissues SummaryWhen the plant tissues were in contact with the highly concentrated sugar solution, the bound water remained in the tissues because it was adsorbed by the protoplasmic colloidal particles; the free water was extravasated into the sugar solution along the water potential gradient because ...
- Experiments for the determination of free water content in plant tissues SummaryWater in plant tissues exists in two different states; bound water that is tightly bound to the protoplasmic colloids, and free water that is not tightly bound to the protoplasmic colloids but is free to move.Operation methodExperiments for the determination of free water content in plant ...
- Experiments for the determination of free proline in plants SummaryPlants grown under normal environmental conditions have low levels of free proline, but under adversity (e.g., drought, cold, salt, etc.) the free proline content in plants can increase 10-100 times, so it has been proposed that the free proline content can be used as an indicator of ...