PTN Mouse mAb: Selection, Handling & Application Guidelines

PTN Mouse mAb is a mouse-derived monoclonal antibody targeting Pleiotrophin (PTN), formulated in PBS and intended for use in standard immunological research applications.

Product SKU

Ab350663

Clone Reference

KC-1700

Host Species

Mouse

Antibody Format

Monoclonal Antibody (mAb)

Formulation

PBS (Phosphate-Buffered Saline) only

Target Antigen

Pleiotrophin (PTN)

Overview of PTN Mouse mAb

Pleiotrophin (PTN), also known as Heparin-Binding Growth-Associated Molecule (HB-GAM), is a secreted growth factor implicated in cell proliferation, differentiation, and angiogenesis. PTN Mouse mAb (Ab350663, clone KC-1700) is a mouse-derived monoclonal antibody raised against PTN and supplied in phosphate-buffered saline (PBS). As a monoclonal antibody, it offers high specificity and lot-to-lot consistency, making it suitable for use as a research tool in immunological assays. Researchers working in oncology, neuroscience, and developmental biology commonly employ anti-PTN antibodies to investigate PTN expression levels and signaling pathways.

Storage, Handling & Stability Guidelines

Proper storage and handling of PTN Mouse mAb are essential to maintain antibody integrity and assay reproducibility. Follow the guidelines below:

• Storage temperature: Store the antibody at –20 °C for long-term storage. Avoid repeated freeze-thaw cycles, as these can denature the antibody and reduce its binding activity.
• Aliquoting: Upon first thaw, prepare single-use aliquots to minimise freeze-thaw cycles. Label each aliquot clearly with date and concentration.
• Formulation note: This product is supplied in PBS only. No carrier proteins (e.g., BSA) or preservatives (e.g., sodium azide) are stated; verify the exact formulation on the Certificate of Analysis (CoA) before use in cell-based assays, as azide is cytotoxic.
• Working solution: Prepare diluted antibody solutions fresh on the day of the experiment in an appropriate assay buffer and keep on ice during use.
• Contamination prevention: Use sterile, low-protein-binding tubes and pipette tips. Avoid microbial contamination of the stock solution.

Recommended Application Protocol

PTN Mouse mAb may be applicable to standard immunoassay techniques commonly used with anti-growth-factor monoclonal antibodies. The following general protocol steps are provided as a starting point; optimisation for your specific system is required:

1. Titration / Optimisation: Determine the optimal working dilution empirically for each application (e.g., Western blot, ELISA, immunofluorescence, immunohistochemistry) using a dilution series. Typical starting ranges for monoclonal antibodies in WB are 1:500–1:2000; for IHC, 1:50–1:200. These are illustrative ranges only — validate experimentally.
2. Sample preparation: Lyse cells or homogenise tissue in an appropriate lysis buffer containing protease inhibitors. For PTN, which is a secreted protein, conditioned media may also serve as a suitable sample type.
3. Blocking: Block non-specific binding with 5% non-fat dry milk or 3–5% BSA in TBST for 1 hour at room temperature, depending on the assay format.
4. Primary antibody incubation: Incubate with the diluted PTN Mouse mAb (Ab350663) at 4 °C overnight or for 1 hour at room temperature, according to the application requirements.
5. Detection: Use an appropriate anti-mouse secondary antibody conjugated to HRP, fluorescent dye, or other reporter, matched to the assay format.
6. Controls: Always include a positive control (PTN-expressing lysate or recombinant PTN), a negative control (non-transfected lysate or isotype control), and, where applicable, a no-primary-antibody control.

Note: Specific validated applications and recommended dilutions for Ab350663 should be confirmed on the product datasheet and CoA available from Aladdin Scientific.

Troubleshooting Common Issues

If unexpected results are observed when using PTN Mouse mAb, consult the troubleshooting guide below:

Problem	Possible Cause	Suggested Action
No signal detected	Antibody degraded; incorrect dilution; incompatible sample type	Check storage conditions; re-titrate antibody; confirm PTN expression in sample with a validated positive control
High background	Insufficient blocking; antibody concentration too high; secondary antibody cross-reactivity	Extend blocking time; reduce primary antibody concentration; use a more stringent wash buffer
Non-specific bands (WB)	Antibody concentration too high; incomplete transfer	Optimise dilution; verify transfer efficiency with a total protein stain
Variable results between experiments	Freeze-thaw damage; reagent lot variation	Aliquot antibody upon receipt; document lot numbers; include inter-assay controls

Quality Control & Ordering Information

PTN Mouse mAb (Ab350663) is manufactured under quality-controlled conditions. Each lot is tested to confirm identity and performance prior to release. Researchers should:

• Request the Certificate of Analysis (CoA) for the specific lot received to confirm tested applications, recommended dilutions, and formulation details.
• Verify the Safety Data Sheet (SDS) prior to handling for hazard classification and safe-disposal information.
• Contact Aladdin Scientific technical support for application-specific guidance or custom requirements.

Ordering details:

• SKU: Ab350663
• Product name: PTN Mouse mAb
• Clone: KC-1700
• Formulation: PBS

Place orders through the Aladdin Scientific online portal or contact your regional sales representative for pricing, availability, and volume discounts.

Frequently asked questions

What applications is PTN Mouse mAb (Ab350663) validated for?

The specific validated applications for Ab350663 should be confirmed on the product datasheet and Certificate of Analysis provided by Aladdin Scientific. Common applications for anti-PTN monoclonal antibodies include Western blot (WB), ELISA, immunofluorescence (IF), and immunohistochemistry (IHC), but empirical optimisation is required for each application and sample type.

How should I store PTN Mouse mAb to ensure maximum stability?

Store PTN Mouse mAb at –20 °C for long-term preservation. Avoid repeated freeze-thaw cycles by aliquoting the antibody into single-use volumes upon first thaw. Keep working dilutions on ice and prepare them fresh on the day of the experiment.

What is the formulation of PTN Mouse mAb Ab350663?

PTN Mouse mAb (Ab350663) is formulated in PBS (phosphate-buffered saline) only. Researchers should consult the Certificate of Analysis for the received lot to confirm the absence or presence of any additional excipients, preservatives, or carrier proteins before use in sensitive assays.

Why am I getting high background with PTN Mouse mAb?

High background is most commonly caused by insufficient blocking, excessively high antibody concentration, or secondary antibody cross-reactivity. To resolve this, extend the blocking step, reduce the primary antibody concentration, increase the number or stringency of wash steps, and ensure the secondary antibody is species-matched and validated for your assay format.

Can PTN Mouse mAb be used to detect PTN in conditioned media?

PTN is a secreted protein, so conditioned cell culture media is a relevant sample type for detecting PTN. Concentration steps such as ultrafiltration or immunoprecipitation may be required prior to detection depending on expression levels. Validate the antibody performance in this sample matrix using appropriate positive and negative controls.

References

1. Aladdin Scientific. PTN Mouse mAb, SKU Ab350663, Clone KC-1700. Product datasheet and Certificate of Analysis. Aladdin Scientific.
2. Deuel TF, Zhang N, Yeh HJ, Silos-Santiago I, Wang ZY. Pleiotrophin: a cytokine with diverse functions and a novel signaling pathway. Arch Biochem Biophys. 2002;397(2):162-171. doi:10.1006/abbi.2001.2705

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.