Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Fucosylation is an important type of glycosylation modification. It refers to the process by which L-fucose is attached to glycan chains of glycoproteins or glycolipids through specific glycosidic linkages. This modification widely participates in cell recognition, receptor signaling, immune ...
The experimental value of lectin-based detection lies in converting glycan recognition into signals that can be localized, compared, and validated. Histochemistry, ELISA, and Western blot correspond to three different levels of information output: spatial distribution analysis, solid-phase ...
Glycosylation is one of the most important post-translational regulatory layers of cell surface molecules. Its role is not limited to increasing the structural complexity of proteins or lipids, but rather lies in continuously reshaping the initiation threshold, transmission efficiency, and ...
In both eukaryotes and prokaryotes, a large proportion of proteins and lipids carry complex oligosaccharide chains in the form of N- or O-linked glycosylation. These glycans participate in key processes such as cell adhesion, signal transduction, immune regulation, and pathogen invasion.
Techniques often explored alongside immunological experiments.
