GAPDH Mouse mAb (Biotin) - Anticorpo primario, specific to GAPDH

Cat. No.: Ab219252

GRADE & PURITY ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry, IF/ICC, WB

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG1

Clone Number

B08/4A11

Conjugation

Biotin

Panoramica

Mouse anti Human GAPDH Antibody, Monoclonal (B08/4A11), could be used for WB; IF/ICC and Flow Cytometry.

Specifications

Product Name

GAPDH Mouse mAb (Biotin) - Anticorpo primario, specific to GAPDH

Sinonimi

38 kDa BFA-dependent ADP-ribosylation substrate antibody | aging associated gene 9 protein antibody | Aging-associated gene 9 protein antibody | BARS-38 antibody | cb609 antibody | EC 1.2.1.12 antibody | Epididymis secretory sperm binding protein Li 162eP

Specifiche e purezza

ExactAb™, Validated, 0.5 mg/mL

Specie ospite

Mouse

Specificità

GAPDH

Immunogeno

Recombinant full length human GAPDH

Controllo positivo

WB: HEK293, HeLa, Jurkat and K562 whole cell lysates Flow Cytometry: HEK293 cells ICC/IF: HeLa cells

Coniugazione

Biotin

Grado

ExactAb™, Validated

Tipo di catena leggera

kappa

Metodo di purificazione

Protein A purified

Concentrazione

0.5 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

Immagini

Western Blot: GAPDH Mouse mAb (Biotin) (Ab219252)

GAPDH Mouse mAb (Biotin) (Ab219252) - Western Blot
All lanes: GAPDH Mouse mAb (Biotin) (Ab219252) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Streptavidin (HRP) (np156148) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 127.5 s

Flow Cytometry: GAPDH Mouse mAb (Biotin) (Ab219252)

GAPDH Mouse mAb (Biotin) (Ab219252) - Flow Cytometry
Flow cytometry analysis of GAPDH expression by HEK293 cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with GAPDH Mouse mAb (Biotin) (Ab219252) (Red). Then incubated with Streptavidin protein (FITC) (rp155876) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

IF/ICC: GAPDH Mouse mAb (Biotin) (Ab219252)

GAPDH Mouse mAb (Biotin) (Ab219252) – IF/ICC
IF analysis of GAPDH (green) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with GAPDH Mouse mAb (Biotin) (Ab219252) at a dilution of 1/1000 in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (Oregon Green 488) (rp217313) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Applicazione

Applicazione

Dilution info

IF/ICC

1/100-1/1000

Flow Cytometry

1/1000 - 1/10000

Obiettivi associati (umani)

GAPDH Tchem Glyceraldehyde-3-phosphate dehydrogenase (0 Activities)

Discover Target: GAPDH

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

How should this product be stored?

Store at ?20 °C, protected from light. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GAPDH Mouse mAb (Biotin)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.