Recombinant GAPDH Antibody - Anticorpo primario, specific to GAPDH

Cat. No.: Ab104781

QUALITÀ & PUREZZA Recombinant, ExactAb™, Validated, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

Coniglio

Reactive species

consiglio, Mouse, Scimmia, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human GAPDH Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.

Specifiche

Product Name

Recombinant GAPDH Antibody - Anticorpo primario, specific to GAPDH

Sinonimi

38 kDa BFA-dependent ADP-ribosylation substrate antibody | aging associated gene 9 protein antibody | Aging-associated gene 9 proteinantibody | BARS-38 antibody | cb609 antibody | EC 1.2.1.12antibody | Epididymis secretory sperm binding protein Li 162eP a

Specifiche e purezza

ExactAb™, Validated, Recombinant, High Performance, See COA

Meccanismi biochimici e fisiologici

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. N

Specie ospite

Coniglio

Specificità

GAPDH

Immunogeno

A synthetic peptide derived from human GAPDH (AA 1-150).

Controllo positivo

WB: HeLa, A431, A549, MCF7, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human bladder cancer tissue. IF/ICC: HeLa cells.

Coniugazione

Unconjugated

Grado

ExactAb™, High Performance, Recombinant, Validated

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant GAPDH Antibody (Ab104781)

Recombinant GAPDH Antibody (Ab104781) - Western Blot
All lanes: Recombinant GAPDH Antibody (Ab104781) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 21.9 s

IHC: Recombinant GAPDH Antibody (Ab104781)

Recombinant GAPDH Antibody (Ab104781) - IHC
Immunohistochemical analysis of paraffin-embedded human bladder cancer,
using Recombinant GAPDH Antibody (Ab104781).

IF/ICC: Recombinant GAPDH Antibody (Ab104781)

Recombinant GAPDH Antibody (Ab104781) - IF/ICC
Immunofluorescent analysis of HeLa using the Recombinant GAPDH Antibody (Ab104781) at 1/50 dilution.

IF/ICC: Recombinant GAPDH Antibody (Ab104781)

Recombinant GAPDH Antibody (Ab104781) - IF/ICC
Immunofluorescent analysis of HeLa using the Recombinant GAPDH Antibody (Ab104781) at 1/50 dilution.

IF/ICC: Recombinant GAPDH Antibody (Ab104781)

Recombinant GAPDH Antibody (Ab104781) - IF/ICC
Immunofluorescent analysis of Hela cells, using Recombinant GAPDH Antibody (Ab104781).

Western Blot: Recombinant GAPDH Antibody (Ab104781)

Recombinant GAPDH Antibody (Ab104781) - Western Blot

All lanes: Recombinant GAPDH Antibody (Ab104781) at 1/100000 dilution

Samples: Lysates at 20 µg per lane

Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution  

Predicted band size: 36 kDa

Observed band size: 35 kDa

Exposure time: 100.0 s

Applicazione

Applicazione

Dilution info

IHC

1/100-1/500

WB

1/1000 - 1/10000

IF/ICC

1/100-1/500

Obiettivi associati (umani)

GAPDH Tchem Glyceraldehyde-3-phosphate dehydrogenase (0 Activities)

Discover Target: GAPDH

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant GAPDH Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.