GNB1 Antibody - Anticorpo primario, specific to GNB1, Rabbit IgG

Cat. No.: Ab105938

GRADE & PURITY Carrier Free, ExactAb™, Validated, 0.2 mg/mL

Application

IF/ICC, IHC, WB

Host species

Coniglio

Reactive species

consiglio, Mouse, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human GNB1 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Specifications

Product Name

GNB1 Antibody - Anticorpo primario, specific to GNB1, Rabbit IgG

Sinonimi

G protein beta subunit GI/GS/GT | guanine nucleotide binding protein (G protein), beta polypeptide 1 | guanine nucleotide-binding protein G | GBB1_HUMAN | Beta subunit signal transducing proteins GS/GI antibody | G protein beta 1 subunit antibody | GBB1 a

Specifiche e purezza

ExactAb™, Validated, Carrier Free, 0.2 mg/mL

Specie ospite

Coniglio

Specificità

GNB1

Immunogeno

Fusion protein of human GNB1 (AA 100-340).

Controllo positivo

WB: Hep G2, A549, HEK293, Jurkat, HeLa, Raji, NIH/3T3 and C6 cell lysates. IHC: Human liver cancer tissue. IF/ICC: HeLa cells

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

0.2 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: GNB1 Antibody (Ab105938)

GNB1 Antibody (Ab105938) - Western Blot
All lanes: GNB1 Antibody (Ab105938) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 37 kDa
Observed band size: 35 kDa
Exposure time: 60.0 s

IHC: GNB1 Antibody (Ab105938)

GNB1 Antibody (Ab105938) - IHC
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using GNB1 Antibody (Ab105938). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: GNB1 Antibody (Ab105938)

GNB1 Antibody (Ab105938) - IF/ICC

IF analysis of GNB1 Antibody (Ab105938) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with GNB1 Antibody (Ab105938) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Applicazione

Applicazione

Dilution info

WB

1/500-1/1000

IHC

1/50 - 1/100

IF/ICC

1/200

Obiettivi associati (umani)

GNB1 Tbio Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (0 Activities)

Discover Target: GNB1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GNB1 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.