Grp75 Antibody - Primary antibody, specific to Grp75, Rabbit IgG

Cat. No.: Ab106524

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.6 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human Grp75 Antibody, Polyclonal antibody, could be used for WB, IHC and so on.

Application:

WB: 1/500-1/1000 IHC: 1/50-1/100

Protein Function

Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.

Specifications

Product Name

Grp75 Antibody - Primary antibody, specific to Grp75, Rabbit IgG

Synonyms

75 kDa glucose regulated protein antibody | 75 kDa glucose-regulated protein antibody | CSA antibody | Glucose Regulated Protein antibody | Grp 75 antibody | GRP-75 antibody | GRP75 antibody | GRP75_HUMAN antibody | Heat shock 70 kDa protein 9 antibody |

Specifications & Purity

ExactAb™, Validated, Carrier Free, 0.6 mg/mL

Host species

Rabbit

Specificity

Grp75

Immunogen

A synthetic peptide derived from human HSPA9 (AA 114-157).

Positive Control

WB: A549, MCF-7, HEK293, HeLa, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

0.6 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Grp75 Antibody (Ab106524)

Grp75 Antibody (Ab106524) - Western Blot
All lanes: Grp75 Antibody (Ab106524) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 75 kDa
Observed band size: 74 kDa
Exposure time: 4.5 s

IHC: Grp75 Antibody (Ab106524)

Grp75 Antibody (Ab106524) - IHC
Immunohistochemistry analysis of paraffin-embedded Human brain using Grp75 Antibody (Ab106524). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Grp75 Antibody (Ab106524)

Grp75 Antibody (Ab106524) - IF/ICC

IF analysis of Grp75 Antibody (Ab106524) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Grp75 Antibody (Ab106524) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

• Application: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

Associated Targets(Human)

HSPA9 Tchem Stress-70 protein, mitochondrial (0 Activities)

Discover Target: HSPA9

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action