GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Panoramica
Rabbit anti Human HMGB2 Antibody, Polyclonal antibody, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
HMGB2 Antibody - Anticorpo primario, specific to HMGB2, Rabbit IgG
Sinonimi
C80539 antibody | High mobility group (nonhistone chromosomal) protein 2 antibody | High mobility group box 2 antibody | High mobility group protein 2 antibody | High mobility group protein B2 antibody | HMG 2 antibody | HMG B2 antibody | HMG-2 antibody |
Specifiche e purezza
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Meccanismi biochimici e fisiologici
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding
Specie ospite
Coniglio
Specificità
HMGB2
Immunogeno
A KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HMGB2.
Controllo positivo
WB: HEL and Jurkat cell lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells
Coniugazione
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Metodo di purificazione
Immunogen affinity purified
Concentrazione
1.0 mg/mL
Product Line
Prodotti biologici
Stoccaggio e spedizione
Forma
Liquid
Condizioni di conservazione di stoccaggio
Store at -20°C,Avoid repeated freezing and thawing
Spedito in
Ice chest + Ice pads
Stabilità e conservazione
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Immagini
HMGB2 Antibody (Ab108314) - Western Blot All lanes: HMGB2 Antibody (Ab108314) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/30000 dilution
Predicted band size: 24 kDa Observed band size: 27 kDa Exposure time: 60.0 s
HMGB2 Antibody (Ab108314) - IHC Immunohistochemical analysis of HMGB2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with HMGB2 Antibody (Ab108314) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
HMGB2 Antibody (Ab108314) - IF/ICC Immunofluorescent analysis of HMGB2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with HMGB2 Antibody (Ab108314) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Applicazione
Applicazione
Dilution info
WB
1/500-1/2000
IF/ICC
1/100-1/500
IHC
1/100-1/200
Documentazione
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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