HMGCS1 Antibody - Anticorpo primario, specific to HMGCS1, Rabbit IgG

Cat. No.: Ab108328

GRADE & PURITY Carrier Free, ExactAb™, Validated, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

Coniglio

Reactive species

Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human HMGCS1 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.01-2µg/mL
IHC: 5-20µg/mL
IF/ICC: 2.5µg/mL
Protein Function
This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

Specifications

Product Name

HMGCS1 Antibody - Anticorpo primario, specific to HMGCS1, Rabbit IgG

Sinonimi

3-hydroxy-3-methylglutaryl coenzyme A synthase | EC 2.3.3.10 | HMG-CoA synthase | 3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble) antibody | 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody | cytoplasmic antibody | EC 2.3.3.10 antibody |

Specifiche e purezza

Carrier Free, ExactAb™, Validated, High Performance, See COA

Specie ospite

Coniglio

Specificità

HMGCS1

Immunogeno

Recombinant HMGCS expressed in E.coli (AA 293-467)

Controllo positivo

WB: HEK293, Hep G2 and HeLa cell lysates. IHC: Human liver, brain, kidney tissues. IF/ICC: HeLa cells.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, High Performance, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: HMGCS1 Antibody (Ab108328)

HMGCS1 Antibody (Ab108328) - Western Blot
All lanes: HMGCS1 Antibody (Ab108328) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 56, 57 kDa
Exposure time: 43.4 s

IHC: HMGCS1 Antibody (Ab108328)

HMGCS1 Antibody (Ab108328) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 µg/mL HMGCS1 Antibody (Ab108328) 
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody. 

IHC: HMGCS1 Antibody (Ab108328)

HMGCS1 Antibody (Ab108328) - IHC
DAB staining on IHC
Samples: Human Brain Tissue
Primary Ab: 10 µg/mL HMGCS1 Antibody (Ab108328)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: HMGCS1 Antibody (Ab108328)

HMGCS1 Antibody (Ab108328) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL HMGCS1 Antibody (Ab108328)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody. 

IF/ICC: HMGCS1 Antibody (Ab108328)

HMGCS1 Antibody (Ab108328) - IF/ICC
IF analysis of HMGCS1 Antibody (Ab108328) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with HMGCS1 Antibody (Ab108328) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

Applicazione

Applicazione

Dilution info

IHC

5-20 µg/mL

WB

0.01-2 µg/mL

IF/ICC

2.5 µg/mL

Obiettivi associati (umani)

HMGCS1 Tchem Hydroxymethylglutaryl-CoA synthase, cytoplasmic (0 Activities)

Discover Target: HMGCS1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (HMGCS1 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.