Hsp60 mAb di topo - Anticorpo primario, specific to Hsp60, Mouse IgG1

Cat. No.: Ab169190

GRADE & PURITY Carrier Free, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

Flow Cytometry, IF/ICC, WB

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG1

Clone Number

EF02/2E10

Conjugation

Unconjugated

Panoramica

Applicazione
WB: 1/1.000 - 1/200.000; peso molecolare previsto: 61,0 KDa
ICC/IF: 1/200
Flusso: 1/800 - 1/200.000

Specifications

Product Name

Hsp60 mAb di topo - Anticorpo primario, specific to Hsp60, Mouse IgG1

Sinonimi

anticorpo chaperonina 60 kDa | Proteina da shock termico 60 kDa, anticorpo mitocondriale | Anticorpo CH60_HUMAN | Chaperonina 60 anticorpo | Chaperonina, 60-KD anticorpo | CPN60 anticorpo | fa04a05 anticorpo | GROEL anticorpo | Heat shock 60kDa protein 1

Specifiche e purezza

Convalidato, ad alte prestazioni, senza carrier, senza azide, ≥95% (SDS-PAGE), 1,0 mg/mL

Specie ospite

Mouse

Specificità

Hsp60

Immunogeno

Recombinant full length protein corresponding to Human Hsp60

Controllo positivo

WB: HEK293, HeLa, HepG2, A549, MOTL-4, MCF-7, Jurkat, K562, A431 and THP-1 Whole cell Lysates ICC/IF: U-2 OS cells Flow: Jurkat cells

Coniugazione

Unconjugated

Grado

Azide Free, Carrier Free, High Performance, PBS Only, Validated

Isotipo

Mouse IgG1

Tipo di catena leggera

kappa

SDS-PAGE

150.0 KDa

Metodo di purificazione

Protein A purified

Concentrazione

1.0 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquido

Condizioni di conservazione di stoccaggio

Conservare a -20°C, evitando di congelare e scongelare ripetutamente

Spedito in

Cassetta del ghiaccio + cuscini per il ghiaccio

Stabilità e conservazione

Conservare a 4°C a breve termine (1-2 settimane). Conservare a -20°C o -80°C. Evitare il ciclo di congelamento/scongelamento.

Immagini

Western Blot: Hsp60 Mouse mAb (Ab169190)

Hsp60 Mouse mAb (Ab169190) - Western Blot
All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 58 kDa
Exposure time: 15.0 s 

Western Blot: Hsp60 Mouse mAb (Ab169190)

Hsp60 Mouse mAb (Ab169190) - Western Blot
All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 4.0 s 

Flow Cytometry: Hsp60 Mouse mAb (Ab169190)

Hsp60 Mouse mAb (Ab169190) - Flow Cytometry
Overlay histogram showing Jurkat cells stained with Hsp60 Mouse mAb (Ab169190) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

ICC/IF: Hsp60 Mouse mAb (Ab169190)

Hsp60 Mouse mAb (Ab169190) - ICC/IF
IF analysis of Hsp60 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp60 Mouse mAb (Ab169190) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Applicazione

Applicazione

Dilution info

IF/ICC

1/200

Flow Cytometry

1/800 - 1/200,000

WB

1/1,000 - 1/200,000; Predicted molecular weight: 61.0 KDa

Obiettivi associati (umani)

HSPD1 Tbio 60 kDa heat shock protein, mitochondrial (0 Activities)

Discover Target: HSPD1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HSPD1 Tbio Heat shock protein HSP 60 (26 Activities)

Discover Target: HSPD1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Meccanismi d'azione

Domande frequenti

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Hsp60 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.