Hsp60 Mouse mAb (Biotin) - Primary antibody, specific to Hsp60

Cat. No.: Ab219266

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry, IF/ICC, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

EF02/2E10

Conjugation

Biotin

Overview

Mouse anti Human Hsp60 Antibody, Monoclonal (EF02/2E10), could be used for WB, IF/ICC and Flow Cytometry.

Specifications

Product Name

Hsp60 Mouse mAb (Biotin) - Primary antibody, specific to Hsp60

Synonyms

60 kDa chaperonin antibody | 60 kDa heat shock protein, mitochondrial antibody | CH60_HUMAN antibody | Chaperonin 60 antibody | Chaperonin, 60-KD antibody | CPN60 antibody | fa04a05 antibody | GROEL antibody | heat shock 60kDa protein 1 (chaperonin) antib

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Mouse

Specificity

Hsp60

Immunogen

Recombinant full length protein corresponding to Human Hsp60

Positive Control

WB: K562 whole cell lysates IF/ICC: Hela cells Flow Cytometry: HEK293 cells

Conjugation

Biotin

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

Images

ICC/IF: Hsp60 Mouse mAb (Biotin) (Ab219266)

Hsp60 Mouse mAb (Biotin) (Ab219266) - ICC/IF
IF analysis of Hsp60 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp60 Mouse mAb (Biotin) (Ab219266) at a dilution of 1/1000 in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (Oregon Green 488) (rp217313)at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Western Blot: Hsp60 Mouse mAb (Biotin) (Ab219266)

Hsp60 Mouse mAb (Biotin) (Ab219266) - Western Blot
All lanes: Hsp60 Mouse mAb (Biotin) (Ab219266) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Streptavidin (HRP) (np156148) at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 68.9 s

Flow Cytometry: Hsp60 Mouse mAb (Biotin) (Ab219266)

Hsp60 Mouse mAb (Biotin) (Ab219266) - Flow Cytometry
Flow cytometry analysis of Hsp60 expression by HEK293 cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with Hsp60 Mouse mAb (Biotin) (Ab219266) (Red). Then incubated with Streptavidin protein (FITC) (rp155876) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• WB:

  1/1000 - 1/10000 ;

• IF/ICC:

  1/100-1/1000 ;

• Flow Cytometry:

  1:50-1:500;

Associated Targets(Human)

HSPD1 Tbio 60 kDa heat shock protein, mitochondrial (0 Activities)

Discover Target: HSPD1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)