MHC class I Mouse mAb (APC) - Anticorpo primario, specific to MHC class I

Cat. No.: Ab189655

GRADE & PURITY Validated, ExactAb™, Ex:650nm, Em:660nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Mouse

Isotype

Mouse IgG2a

Clone Number

34-1-2S

Conjugation

APC

Panoramica

Mouse anti Mouse MHC class I Antibody, Monoclonal (34-1-2S), could be used for Flow, in vivo activation of APCs and so on.

Specifications

Product Name

MHC class I Mouse mAb (APC) - Anticorpo primario, specific to MHC class I

Sinonimi

H-2D | H2-D | H2-K1 | K-f | H-2K | H2-K | H2-D1 | H-2K(d) | A-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alph

Specifiche e purezza

ExactAb™, Validated, Ex:650nm, Em:660nm, 0.5 mg/mL

Specie ospite

Mouse

Specificità

MHC class I

Immunogeno

BDF mouse spleen cells

Controllo positivo

Flow: C57BL/6 mouse splenocytes.

Coniugazione

APC

Eccitazione (nm)

Red Laser(633nm)

Emissione (nm)

660nm

Ex/Em(nm)

Ex:650nm;Em:660nm

Grado

Em:660nm, Ex:650nm, ExactAb™, Validated

Tipo di catena leggera

kappa

Metodo di purificazione

Protein G purified

Concentrazione

0.5 mg/mL

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at 2-8°C,Protected from light,Do not freeze

Spedito in

Wet ice,Do not freeze

Stabilità e conservazione

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Immagini

Flow Cytometry: MHC class I Mouse mAb (APC) (Ab189655)

MHC class I Mouse mAb (APC) (Ab189655) - Flow Cytometry
Flow cytometry analysis of MHC class I expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with MHC class I Mouse mAb (APC) (Ab189655) at 0.2μg/test for 1 hour at 4°C. Left panel - Mouse IgG (APC) (Ab180309). 

Flow Cytometry: MHC class I Mouse mAb (APC) (Ab189655)

MHC class I Mouse mAb (APC) (Ab189655) - Flow Cytometry
Flow cytometry analysis of MHC class I expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with MHC class I Mouse mAb (APC) (Ab189655) at 0.2μg/test for 1 hour at 4°C. Blue - Mouse IgG (APC) (Ab180309). Black - Unlabelled control, cells without incubation with primary antibody.

Applicazione

• Applicazione: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume ;

Obiettivi associati (umani)

HLA-A Tbio HLA class I histocompatibility antigen, A-31 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MHC class I Mouse mAb (APC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.