MTHFD2 mAb di topo - Anticorpo primario, specific to MTHFD2, Mouse IgG1

Cat. No.: Ab116276

QUALITÀ & PUREZZA Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, IP, WB

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG1

Clone Number

K70015_2H12

Conjugation

Unconjugated

Panoramica

L'anticorpo di topo anti MTHFD2 umano, monoclonale (K70015_2H12), può essere utilizzato per WB, IHC, IP e così via.
Applicazione:
WB: 1/1000
IHC: 1/200
IP: 1/100

Specifiche

Product Name

MTHFD2 mAb di topo - Anticorpo primario, specific to MTHFD2, Mouse IgG1

Sinonimi

NMDMC | MTHFD2 | Metilenetetraidrofolato deidrogenasi/cicloidrolasi bifunzionale, anticorpo mitocondriale | Descrizione anticorpo | Meteniltetraidrofolato cicloidrolasi anticorpo | Metilenetetraidrofolato deidrogenasi (NAD+ dipendente) anticorpo |

Specifiche e purezza

Privo di carrier, ExactAb™, convalidato, 1,0 mg/mL

Specie ospite

Mouse

Specificità

MTHFD2

Immunogeno

Recombinant protein of human MTHFD2 (AA 101-300)

Controllo positivo

WB: A431, Hep G2, HeLa, HEK293 and MOLT-4 cell lysates. IHC: Human kidney tissue. IP: A431 lysates.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

1.0 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquido

Condizioni di conservazione di stoccaggio

Conservare a -80°C, evitando di congelare e scongelare ripetutamente

Spedito in

Dry ice packs + Cold packs

Stabilità e conservazione

Conservare a 2-8 ℃ (3 mesi). Conservare a -20 ℃ (12 mesi). Conservare a -80 ℃ (36 mesi). Al momento del ricevimento, si consiglia di effettuare un'aliquota. Evitare il ciclo di congelamento/scongelamento.

Immagini

Western Blot: MTHFD2 Mouse mAb (Ab116276)

MTHFD2 Mouse mAb (Ab116276) - Western Blot
All lanes: MTHFD2 Mouse mAb (Ab116276) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 34 kDa
Exposure time: 169.7 s

IP: MTHFD2 Mouse mAb (Ab116276)

MTHFD2 Mouse mAb (Ab116276) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of MTHFD2 Mouse mAb (Ab116276) with A431 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. MTHFD2Mouse mAb (Ab116276) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Lane 1: A431 lysateLane 2: MTHFD2 immunoprecipitated from A431 lysate by MTHFD2 Mouse mAb (Ab116276) 
Lane 3: IgG isotype control antibody instead of MTHFD2 Mouse mAb (Ab116276) in A431 lysate.
Result: MTHFD2 Mouse mAb (Ab116276) can immunoprecipitate MTHFD2.

IHC: MTHFD2 Mouse mAb (Ab116276)

MTHFD2 Mouse mAb (Ab116276) - IHC
IHC-P analysis of kidney tissue by MTHFD2 Mouse mAb (Ab116276).IHC-P was performed using sections of the formalin-fixed paraffin-embedded kidney tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H₂O₂ for 30 min at room temperature. The sections were then incubated with MTHFD2 Mouse mAb (Ab116276) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in tubules are positively stained at the cytoplasm.

Applicazione

Applicazione

Dilution info

WB

1/1000

IP

1/100

IHC

1/200

Obiettivi associati (umani)

MTHFD2 Tchem Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial (0 Activities)

Discover Target: MTHFD2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MTHFD2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.