Phosphatase, Alkaline, CAS No.9001-78-9

CAS: 9001-78-9 Cat. No.: P128629 Numero EC: 232-631-4 PubChem CID: 18985873

QUALITÀ & PUREZZA Native, EnzymoPure™, ≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

Accession #

P00634

Bioactivity

≥30 units/mg protein (25°C, pH 8.0)

Panoramica

Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum.
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required.Inibibitors: The enzyme is inhibited by chelating agents and inorganic phosphates.

Unit Definitons

E. coli. One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.

Specifiche

Product Name

Phosphatase, Alkaline, CAS No.9001-78-9

Sinonimi

Alkaline phosphatase | Phosphatase, alkaline | ENTEROSTATIN,HUMAN | alkaline phosphomonoesterase | glycerophosphatase | phosphomonoesterase | Orthophosphoric-monoester phosphohydrolase (alkaline optimum) | alkaline phosphatase | Apase, Escherichia coli |

Grado

Native, EnzymoPure™

Specifiche e purezza

EnzymoPure™, Nature, ≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

Meccanismi biochimici e fisiologici

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad

Bioattività

≥30 units/mg protein (25°C, pH 8.0)

Numero d'ordine

P00634

CAS

9001-78-9

Numero della Commissione Enzimi

I.U.B.: 3.1.3.1

Tipo di molecola

Enzima

Stoccaggio e spedizione

Concentrazione

≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

Condizioni di conservazione di stoccaggio

Store at 2-8°C

Spedito in

Wet ice

Definizione dell'unità

One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.