Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Tyramide Signal Amplification (TSA) is one of the most representative enhancement strategies currently used in in situ fluorescence detection. Its core value is not simply to increase fluorescence intensity, but to convert local signals around low-abundance targets into a high-density, spatially ...
Colorimetric means the reaction generates a colored product. You can judge color intensity by eye, or quantify it by measuring absorbance (OD, optical density) with an instrument. In general, deeper color indicates more colored product formed → a stronger target signal (within an appropriate ...
When choosing a secondary antibody for western blotting, one of the main selection criteria is the species of the primary antibody that the secondary antibody binds to.
Horseradish Peroxidase (HRP) is an enzyme widely used in biochemical experiments. With its ability to catalyze the decomposition of hydrogen peroxide, HRP generates detectable products through oxidation-reduction reactions involving substrates.
The basic principles and operation steps of Western Blot experiments of histones are similar to those of other conventional proteins, both of which rely on specific antibodies to detect the presence and expression of target proteins. However, due to the special properties and functions of ...
Western blotting is a technique that uses specific antibodies to separate proteins and is widely used to analyze the expression of specific proteins in cell or tissue extracts. This protocol details the steps for performing western blotting of cell culture and tissue samples using both ...
Techniques often explored alongside immunological experiments.
