Protease from Bacillus licheniformis, CAS No.9014-01-1

CAS: 9014-01-1 Cat. No.: P298986 Numero EC: 232-752-2

GRADE & PURITY EnzymoPure™, ≥2.4 U/g

Accession #

P00780

Bioactivity

≥2.4 AU-A/g

Panoramica

Proteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases.

Application

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations.

The enzyme has been used to prepare chicken (leg) bone protein hydrolysates while evaluating ACE inhibitory peptides.

It has also been used for the hydrolysis of Chlorella pyrenoidosa protein extracts during a study of their antitumor activities.

Biochem/physiol Actions

This product is an endo-protease of the serine type. It has a broad substrate specificity and can hydrolyze most peptide bonds within a protein molecule. It is active between pH 6.5 and 8.5 and has an optimum temperature of 60 °C. The enzyme is is used in detergent formulations to remove protein-based stains.

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Composition (w/w)

Glycerol, CAS no. 56-81-5: 50%

Water, CAS no. 7732-18-5: 41%

Protease (Subtilisin), CAS no. 9014-01-1*: 9%

* Defined as enzyme concentration (dry matter basis)

Allergens

Allergen	Contains substance¹	Allergen	Contains substance¹
Celery	No	Molluscs	No
Gluten-containing cereals²⁴	No	Mustard	No
Crustaceans	No	Nuts³	No
Eggs	No	Peanuts	No
Fish	No	Sesame seeds	No
Lupin	No	Soybeans	No
Milk (including lactose)	No	Sulphur dioxide/sulphites >10 mg/kg or 10 mg/l	No

¹ The definitions of substances follow the requirements of the revised EU Labelling Regulation 1169/2011. The list includes allergen types mentioned in 21 USC 301 (USA) and GB7718-2011 (China).

² Examples: wheat, rye, barley, oats, spelt, kamut

³ Examples: almonds, hazelnuts, walnuts, cashews, pecans, Brazil nuts, pistachios, macadamia nuts and Queensland nuts

⁴ "No" means: gluten-free, i.e., < 20 ppm (according to EU Regulation 828/2014)

Nutritional Information

The main nutritional value of this product is approximately 650 kJ/100 g enzyme product.

Protein: 9 g/100 g

Polyhydroxy compounds: 50 g/100 g

Moisture: 41 g/100 g

GMO Status

This product is not a genetically modified organism.

Production strain name: Bacillus licheniformis

The enzyme preparation is produced by microbial fermentation, and the microorganism is not present in the final product. The microorganism used for production has not been modified using modern biotechnology.

Safety and Handling Precautions

Enzymes are proteins. Inhalation of dust or aerosols may cause sensitization and allergic reactions in sensitized individuals. Some enzymes may irritate the skin, eyes and mucous membranes with prolonged contact.

Sequence similarity: Belongs to the peptidase S8 family

Specifications

Product Name

Protease from Bacillus licheniformis, CAS No.9014-01-1

Sinonimi

[email protected] | Subtilisin A | Subtilisin Carslberg | Proteinase from Bacillus licheniformis | Subtilo peptidase A | Alkaline Protease | subtilisin | subC

Grado

EnzymoPure™

Specifiche e purezza

EnzymoPure™, ≥2.4 U/g

Meccanismi biochimici e fisiologici

Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Shows high specificity for aromatic and hydrophobic amino acids in the P1 substrate position. May play an important role in the degradatio

Bioattività

≥2.4 AU-A/g

Numero d'ordine

P00780

CAS

9014-01-1

Numero della Commissione Enzimi

3.4.21.62

Tipo di molecola

Enzima

Informazioni legali

Alcalase is a registered trademark of Novozymes Corp.

Stoccaggio e spedizione

Concentrazione

≥2.4 U/g

Condizioni di conservazione di stoccaggio

Store at 2-8°C

Spedito in

Wet ice

Stabilità e conservazione

Store at 2-8℃ long term (24 months). Upon receipt, it is recommended to aliquot. Store in the dark. Desiccated.

Definizione dell'unità

AU‑A (Alcalase Unit‑Anson): The amount of enzyme that releases 1 μmol tyrosine per minute from denatured hemoglobin at pH 7.5 and 25 °C.