Protease Subtilisin A, CAS No.9014-01-1

CAS: 9014-01-1 Cat. No.: P755356 Numero EC: 232-752-2

GRADE & PURITY Native, EnzymoPure™, from Bacillus licheniformis;>8 U/mg protein

Accession #

P00780

Bioactivity

>8 U/mg protein

Panoramica

High purity Protease (Subtilisin A from Bacillus licheniformis) (Powder) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

PROPERTIES

1. ELECTROPHORETIC PURITY:

- Single major band on SDS-PAGE (MW = 30,250); minor bands at MW < 30,000

- Single major band on isoelectric focusing (pI 8.8); minor band at pI 9.3

2. PHYSICOCHEMICAL PROPERTIES:

pH Optima: 7.0-7.5 (60% of maximal activity at pH 6.0)

pH Stability: 5.5-10.0

Temperature Optima: 60°C

Temperature Stability: < 60°C

This enzyme is suitable for use in AOAC INTERNATIONAL Total Dietary Fiber analytical procedures and is recommended for AOAC 2001.03.

Specifications

Product Name

Protease Subtilisin A, CAS No.9014-01-1

Sinonimi

Proteinase from Bacillus licheniformis | Subtilisin A | Subtilisin Carslberg | subtilisin | subC

Grado

Native, EnzymoPure™

Specifiche e purezza

EnzymoPure™, Nature, from Bacillus licheniformis;>8 U/mg protein

Meccanismi biochimici e fisiologici

Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Shows high specificity for aromatic and hydrophobic amino acids in the P1 substrate position. May play an important role in the degradatio

Bioattività

>8 U/mg protein

Numero d'ordine

P00780

CAS

9014-01-1

Numero della Commissione Enzimi

3.4.21.62

Tipo di molecola

Enzima

Product Line

Prodotti biologici

Stoccaggio e spedizione

Concentrazione

from Bacillus licheniformis;>8 U/mg protein

Ricostituzione

Accurately weigh 2.5g of protease powder and prepare a solution containing 50 mg of protease per milliliter using 50 mL of 50 mmol/L maleic acid buffer. Vortex and shake for 5 minutes before use. Store at 4 ℃ before use.

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at -20℃ long term (＞4 years).

Definizione dell'unità

One Unit will hydrolyse casein to produce colour equivalent to one μmole (181 μg) of tyrosine per minute at pH 7.0 at 40°C (colour by Folin-Ciocalteu reagent).