Protease Subtilisin A, CAS No.9014-01-1

CAS: 9014-01-1 Cat. No.: P755356 EC Number: 232-752-2

GRADE & PURITY Native, EnzymoPure™, from Bacillus licheniformis;>8 U/mg protein

Accession #

P00780

Bioactivity

>8 U/mg protein

Overview

High purity Protease (Subtilisin A from Bacillus licheniformis) (Powder) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

PROPERTIES

1. ELECTROPHORETIC PURITY:

- Single major band on SDS-PAGE (MW = 30,250); minor bands at MW < 30,000

- Single major band on isoelectric focusing (pI 8.8); minor band at pI 9.3

2. PHYSICOCHEMICAL PROPERTIES:

pH Optima: 7.0-7.5 (60% of maximal activity at pH 6.0)

pH Stability: 5.5-10.0

Temperature Optima: 60°C

Temperature Stability: < 60°C

This enzyme is suitable for use in AOAC INTERNATIONAL Total Dietary Fiber analytical procedures and is recommended for AOAC 2001.03.

Specifications

Product Name

Protease Subtilisin A, CAS No.9014-01-1

Synonyms

Proteinase from Bacillus licheniformis | Subtilisin A | Subtilisin Carslberg | subtilisin | subC

Grade

Native, EnzymoPure™

Specifications & Purity

EnzymoPure™, Nature, from Bacillus licheniformis;>8 U/mg protein

Biochemical and Physiological Mechanisms

Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Shows high specificity for aromatic and hydrophobic amino acids in the P1 substrate position. May play an important role in the degradatio

Bioactivity

>8 U/mg protein

Accession #

P00780

CAS

9014-01-1

Enzyme Commission Number

3.4.21.62

Molecule Type

Enzyme

Storage and Shipping

Concentration

from Bacillus licheniformis;>8 U/mg protein

Reconstitution

Accurately weigh 2.5g of protease powder and prepare a solution containing 50 mg of protease per milliliter using 50 mL of 50 mmol/L maleic acid buffer. Vortex and shake for 5 minutes before use. Store at 4 ℃ before use.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (＞4 years).

Unit definition

One Unit will hydrolyse casein to produce colour equivalent to one μmole (181 μg) of tyrosine per minute at pH 7.0 at 40°C (colour by Folin-Ciocalteu reagent).

References

1. Jianjun Guo, Liang Yao, Xianqing Wang, Rijian Song, Bo Yang, Daochao Jin, Jianjun Guo, Guohua Wu.  (2024)  Dual-Responsive Antibacterial Hydrogel Patch for Chronic-Infected Wound Healing.  BIOMACROMOLECULES,      [PMID:39418536] [10.1021/acs.biomac.4c00981]

2. Changrong Wang, Mengfan Lin, Yibin Li, Weijing Zhuang, Zebin Guo.  (2024)  Effect of steam explosion modified soluble dietary fiber from Tremella fuciformis stem on the quality and digestibility of biscuits.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:38492690] [10.1016/j.ijbiomac.2024.130905]

3. Likun Cheng, Shuang Qu, Yueying Yun, Yan Ren, Fucheng Guo, Yakun Zhang, Guoze Wang.  (2024)  Effects of different thermal processing methods on amino acid, fatty acid, and volatile flavor substance contents of Aohan millet (Golden seedling millet).  Food Science & Nutrition,      [PMID:39620021] [10.1002/fsn3.4409]

4. Xiang Zou, Junguo He, Xinlei Pan, Qiupeng Cai, Shengye Duan, Xinxin Cui, Yijie Zhong, Jie Zhang.  (2024)  Lysozyme coupling protease pretreatment to relieve the humic acid inhibition on excess sludge anaerobic fermentation.  BIORESOURCE TECHNOLOGY,      [PMID:39396577] [10.1016/j.biortech.2024.131626]

5. Qianxia Lin, Siyi Song, Jinfeng Pei, Leifang Zhang, Xiaoe Chen, Huoxi Jin.  (2024)  Preparation and characterization of cysteine-rich collagen peptide and its antagonistic effect on microplastic induced damage to HK-2 cells.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2024.104647]

6. Mengfan Lin, Changrong Wang, Wenfei Wu, Qingsong Miao, Zebin Guo.  (2024)  Quality, improvement of soluble dietary fiber from Dictyophora indusiata by-products by steam explosion and cellulase modification: Structural and functional analysis.  Food Chemistry-X,      [PMID:39791116] [10.1016/j.fochx.2024.102084]

7. Anqi Wang, Xuanyu Wang, Min Zhuang, Sheng Ke, Ming Ning, Peng Yu, Zhongkai Zhou.  (2024)  Size-dependent physicochemical property and functionality of insoluble dietary fiber derived from wheat bran.  LWT-FOOD SCIENCE AND TECHNOLOGY,      [PMID:] [10.1016/j.lwt.2024.115747]

8. Simin Feng, Shuqi Liang, Jianyu Yang, Jiahao Yu, Yang Lin, Yizhen Xie, Peilong Sun, Ping Shao.  (2025)  Naringenin-loaded Flammulina velutipes chitin-glucan complexes gel system: structural modulation and dual lipid-lowering mechanisms.  FOOD CHEMISTRY,      [PMID:40774213] [10.1016/j.foodchem.2025.145791]

9. Xue Wu, Yunhe Chang, Mingfa Song, Juncai Hou, Hongxia Feng.  (2025)  Microencapsulation of Camellia oleifera Seed Oil Emulsion By-Products: Structural Characterization and Lipidomics Analysis.  Foods,  14  (19): (3314).  [PMID:41097485] [10.3390/foods14193314]

10. Jinxin Li, Hao Zhang, Ling Zhu, Gangcheng Wu, Hui Zhang.  (2023)  Influence of in vitro gastrointestinal digestion and colonic fermentation on carbonyl scavenging capacity of fiber-bound polyphenols from quinoa.  Food & Function,  14  (23): (10581-10590).  [PMID:37955444] [10.1039/D3FO03000H]

11. Changrong Wang, Mengfan Lin, Yibin Li, Zebin Guo.  (2023)  Improvement of soluble dietary fiber quality in Tremella fuciformis stem by steam explosion technology: An evaluation of structure and function.  FOOD CHEMISTRY,      [PMID:37924764] [10.1016/j.foodchem.2023.137867]

12. Xiang Zou, Junguo He, Heliang Pang, Pengfei Zhang, Xinlei Pan, Yijie Zhong, Shengye Duan, Xinxin Cui, Xiaodong Xin, Jie Zhang.  (2023)  Investigating the synergic role of asynchronous dosed protease and lysozyme for facilitating excess sludge solubilization and acidogenic fermentation.  SCIENCE OF THE TOTAL ENVIRONMENT,      [PMID:37146803] [10.1016/j.scitotenv.2023.163759]

13. Xiang Zou, Junguo He, Pengfei Zhang, Xinlei Pan, Yijie Zhong, Jie Zhang, Xuewei Wu, Biqing Li, Xia Tang, Xiannian Xiao, Heliang Pang.  (2022)  Insights into carbon recovery from excess sludge through enzyme-catalyzing hydrolysis strategy: Environmental benefits and carbon-emission reduction.  BIORESOURCE TECHNOLOGY,      [PMID:35304256] [10.1016/j.biortech.2022.127006]

14. Yan Zhang, Bi-Cong Wang, Po Wang, Xiao-Jie Ju, Mao-Jie Zhang, Rui Xie, Zhuang Liu, Wei Wang, Liang-Yin Chu.  (2021)  Microfluidic fabrication of hydrogel microparticles with MOF-armoured multi-enzymes for cascade biocatalytic reactions.  Reaction Chemistry & Engineering,  7  (2): (275-283).  [PMID:] [10.1039/D1RE00257K]

15. Zhai Xinyu, Ao Huanping, Liu Wenhui, Zheng Jiaxin, Li Xingjie, Ren Difeng.  (2021)  Physicochemical and structural properties of dietary fiber from Rosa roxburghii pomace by steam explosion.  JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE,  59  (6): (2381-2391).  [PMID:35602434] [10.1007/s13197-021-05254-7]

16. Yaqi Hou, Jiao Liu, Yanchun Shao, Xitian Peng, Dawen Zhang, Lifang Hu, Fusheng Chen, Youxiang Zhou.  (2021)  Evaluation of the underestimation of citrinin content in Hongqu using hydrolysis treatments and UPLC-FLD.  FOOD CONTROL,      [PMID:] [10.1016/j.foodcont.2021.108245]

17. Xingjie Zhang, Kexue Zhu, Jianhua Xie, Yi Chen, Lehe Tan, Shuai Liu, Ruihong Dong, Yuting Zheng, Qiang Yu.  (2020)  Optimization and identification of non-extractable polyphenols in the dietary fiber of jackfruit (Artocarpus heterophyllus Lam.) pulp released by alkaline, acid and enzymatic hydrolysis: Content, composition and antioxidant activities.  LWT-FOOD SCIENCE AND TECHNOLOGY,      [PMID:] [10.1016/j.lwt.2020.110400]

18. Tao Yang, Hui-Ling Yan, Chuan-He Tang.  (2020)  Wet media planetary ball milling remarkably improves functional and cholesterol-binding properties of okara.  FOOD HYDROCOLLOIDS,      [PMID:] [10.1016/j.foodhyd.2020.106386]

19. Weijie Wu, Jie Hu, Haiyan Gao, Hangjun Chen, Xiangjun Fang, Honglei Mu, Yanchao Han, Ruiling Liu.  (2020)  The potential cholesterol-lowering and prebiotic effects of bamboo shoot dietary fibers and their structural characteristics.  FOOD CHEMISTRY,      [PMID:32615381] [10.1016/j.foodchem.2020.127372]

20. Yan Wang, Xiao Kong, Mengdi Du, Yang Qiao, Minjie Ye, Bing Han, Jing Gan.  (2024)  Preparation, Identification, and Digestive Stability of Antioxidant Peptides From Chlorella vulgaris.  JOURNAL OF FOOD BIOCHEMISTRY,  2024  (1): (3828251).  [PMID:] [10.1155/2024/3828251]

21. Jing Yang, Jing Shi, Ying Zhou, Ye Zou, Weimin Xu, Xiudong Xia, Daoying Wang.  (2024)  Preparation, Characterization and Stability of Calcium-Binding Peptides Derived from Chicken Blood.  Foods,      [PMID:39123559] [10.3390/foods13152368]

22. Yuan Guo, Baoyi Wang, Xiaona Zhai, Liang Liang, Jianxiong Hao, Haisheng Pei, Yuanyuan Li.  (2025)  Insights into improvement of the physicochemical and structural properties of wheat bran powder via combined effects of superfine grinding and glutenin.  JOURNAL OF CEREAL SCIENCE,      [PMID:] [10.1016/j.jcs.2025.104140]

23. Xiaoqin Ding, Yabin Zhao, Renlin Zheng, Jimeng Cai, Xinyu Peng, Liangchun Li.  (2025)  Enhanced antioxidant peptide extraction from zein using optimized ultrasound-assisted dual-enzyme methods hydrolysis.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:40203933] [10.1016/j.ijbiomac.2025.142938]

24. Weifan Gao, Yan Cui, Mingwei Xue, Xintao Liu, Xin Meng, Wutong Jiang, Xinyu Jiang, Dali Zhang, Mingzhu Zheng, Jingsheng Liu.  (2025)  Study on the ultrasonic treatment of black rice dietary fiber and its effect on anthocyanin adsorption.  ULTRASONICS SONOCHEMISTRY,      [PMID:40451112] [10.1016/j.ultsonch.2025.107409]

25. Dan Wang, Xiao-Tong Zhai, Na-Na Wu, Jun Li, Ming Zhao, Na Zhang, Bin Tan.  (2025)  Influence of solid-state fermentation with lactic acid bacteria on the structure, physicochemical and adsorption properties of dietary fiber in rice bran.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2025.107869]

26. Jing Liang, Jiayan Xie, Yue Gu, Jianhua Xie, Yi Chen, Bing Zheng, Yue Qiu, Xiaobo Hu, Qiang Yu.  (2025)  Optimization and identification of bound polyphenols from rice bean (Vigna umbellata) skin dietary fiber, antioxidant activities and α-glucosidase inhibition mechanism.  Food Chemistry-X,      [PMID:41323664] [10.1016/j.fochx.2025.103278]

27. Long Sun, Yiqing Jia, Min Jiang, Renfeng Ye, Zhengxuan Wang, Guiguang Cheng, Yaping Liu.  (2025)  Tailoring purple rice bran protein via multi-modal modifications: Chemical, physical, and enzymatic strategies for enhanced functionality.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:41271049] [10.1016/j.ijbiomac.2025.149047]

28. Li Mengyuan, Li Hang, Zhang Manman, Wang Tong, Wang Yongsheng, Wang Zhihan, Wang Shihan.  (2026)  Ultrasound-assisted enzymatic extraction of Rana chensinensis ovum oil and optimization using response surface methodology.  JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE,      [PMID:] [10.1007/s13197-026-06619-6]