GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human RAD17 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on.
Specifications
Product Name
RAD17 Antibody - Primary antibody, specific to RAD17, Rabbit IgG
Synonyms
CCYC antibody | Cell cycle checkpoint protein antibody | Cell cycle checkpoint protein RAD17 antibody | FLJ41520 antibody | HRAD 17 antibody | hRad17 antibody | R24L antibody | Rad 17 antibody | Rad 24 antibody | RAD1 (S. pombe) homolog antibody | RAD1 ho
Specifications & Purity
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Biochemical and Physiological Mechanisms
Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the 9-1-1 (RAD1-RAD9-HUS1)
Host species
Rabbit
Specificity
RAD17
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RAD17.
Positive Control
WB: K562, Jurkat and MDA-MB-231 cell lysates. IHC: Human lung cancer tissue. IF/ICC: A549 cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
RAD17 Antibody (Ab191358) - Western Blot All lanes: RAD17 Antibody (Ab191358) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution Predicted band size: 77 kDa Observed band size: 79 kDa Exposure time: 48.5 s
RAD17 Antibody (Ab191358) - IHC Immunohistochemical analysis of RAD17 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with RAD17 Antibody (Ab191358) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
RAD17 Antibody (Ab191358) - IF/ICC Immunofluorescent analysis of RAD17 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with RAD17 Antibody (Ab191358) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Application
Application
Dilution info
WB
1/500-1/1000
IHC
1/100-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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