RBM4 Antibody - Anticorpo primario, specific to RBM4, Rabbit IgG

Cat. No.: Ab124789

GRADE & PURITY Carrier Free, ExactAb™, Validated, 0.5 mg/mL

Application

WB

Host species

Coniglio

Reactive species

Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human RBM4 Antibody, Polyclonal, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function:
RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of EIF2C2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.

Specifications

Product Name

RBM4 Antibody - Anticorpo primario, specific to RBM4, Rabbit IgG

Sinonimi

hLark | Lark homolog | RNA-binding motif protein 4 | RNA-binding motif protein 4a | dkfzp547k0918 antibody | hLark antibody | lark antibody | Lark homolog antibody | lark homologue antibody | mgc75138 antibody | RBM4 antibody | rbm4 lark antibody | RBM4_H

Specifiche e purezza

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Specie ospite

Coniglio

Specificità

RBM4

Immunogeno

A KLH conjugated synthetic peptide from the C-terminal region of human RBM4 (AA 334-362).

Controllo positivo

WB: MDA-MB-231, A375, HEK293 and NIH/3T3 cell lysates.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified + Peptide affinity purified

Concentrazione

0.5 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: RBM4 Antibody (Ab124789)

RBM4 Antibody (Ab124789) - Western Blot
All lanes: RBM4 Antibody (Ab124789) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 40 kDa
Observed band size: 38 kDa
Exposure time: 60.0 s

Western Blot: RBM4 Antibody (Ab124789)

RBM4 Antibody (Ab124789) - Western Blot
All lanes: RBM4 Antibody (Ab124789) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 40 kDa
Observed band size: 16 kDa
Exposure time: 60.0 s

Applicazione

Applicazione

Dilution info

WB

1/500-1/1000

Obiettivi associati (umani)

RBM4 Tbio RNA-binding protein 4 (0 Activities)

Discover Target: RBM4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RBM4 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.