RCAS1 Mouse mAb - Anticorpo primario, specific to RCAS1, Mouse IgG1

Cat. No.: Ab124859

QUALITÀ & PUREZZA Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, WB

Host species

Mouse

Reactive species

consiglio, Mouse, Umano

Isotype

Mouse IgG1

Clone Number

K40051_4H7

Conjugation

Unconjugated

Panoramica

Mouse anti Human RCAS1 Antibody, Monoclonal (K40051_4H7), could be used for WB, IHC and so on.
Application:
WB:1/1000
IHC:1/100
Protein Function:
May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.

Specifiche

Product Name

RCAS1 Mouse mAb - Anticorpo primario, specific to RCAS1, Mouse IgG1

Sinonimi

PDAF antibody | RCAS 1 antibody | RCAS1 antibody | RCAS1_HUMAN antibody | Receptor binding cancer antigen expressed on SiSo cells antibody | Receptor-binding cancer antigen expressed on SiSo cells antibody | EBAG9 | EB9 | PDAF | BAG9 antibody | Cancer ass

Specifiche e purezza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Specie ospite

Mouse

Specificità

RCAS1

Immunogeno

Recombinant human EBAG9 (AA 41-210).

Controllo positivo

WB: SK-OV-3, MCF7, A549, Jurkat, HEK293, A431, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue.

Coniugazione

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

1.0 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -80°C,Avoid repeated freezing and thawing

Spedito in

Dry ice packs + Cold packs

Stabilità e conservazione

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: RCAS1 Mouse mAb (Ab124859)

RCAS1 Mouse mAb (Ab124859) - Western Blot
All lanes: RCAS1 Mouse mAb (Ab124859) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 24 kDa
Observed band size: 31 kDa
Exposure time: 36.5 s

IHC: RCAS1 Mouse mAb (Ab124859)

RCAS1 Mouse mAb (Ab124859) - IHC
IHC analysis of human brain tissue by RCAS1 Mouse mAb (Ab124859). IHC was performed using sections of the formalin-fixed paraffin-embedded human brain tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with RCAS1 Mouse mAb (Ab124859) at 10 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Glial cells and neuronal cells are positively stained at the cytoplasm.

Applicazione

Applicazione

Dilution info

WB

1/1000

IHC

1/100

Obiettivi associati (umani)

EBAG9 Tbio Receptor-binding cancer antigen expressed on SiSo cells (0 Activities)

Discover Target: EBAG9

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RCAS1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.