Recombinant beta Casein Antibody - Anticorpo primario, specific to beta Casein

Cat. No.: Ab091057

QUALITÀ & PUREZZA Recombinant, ExactAb™, Validated, See COA

Application

WB

Host species

Coniglio

Reactive species

Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human beta Casein Antibody, Recombinant, could be used for WB and so on.

Specifiche

Product Name

Recombinant beta Casein Antibody - Anticorpo primario, specific to beta Casein

Sinonimi

Beta-casein antibody | CASB antibody | CASB_HUMAN antibody | Casein beta antibody | CSN 2 antibody | Csn2 antibody | CSN2 | CASB | PDC213

Specifiche e purezza

Recombinant, ExactAb™, Validated, See COA

Meccanismi biochimici e fisiologici

Important role in determination of the surface properties of the casein micelles.

Specie ospite

Coniglio

Specificità

beta Casein

Immunogeno

A synthetic peptide derived from human beta Casein (AA 100-150).

Controllo positivo

WB: Human milk lysates.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant beta Casein Antibody (Ab091057)

Recombinant beta Casein Antibody (Ab091057) - Western Blot
All lanes: Recombinant beta Casein Antibody (Ab091057) at 1/1000 dilution
Samples: Lysates at 20 µl(1/50 dilution) per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 27 kDa
Exposure time: 50.0 s

Applicazione

Applicazione

Dilution info

WB

1/1000-1/2000

Obiettivi associati (umani)

CSN2 Tbio Beta-casein (0 Activities)

Discover Target: CSN2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Only product-provided application details may be listed here. For this item, specific application protocols, tested assay types (WB, ELISA, IHC, IF, IP), recommended dilutions, incubation times, and positive controls are not specified; refer to CoA/Spec Sheet.

Users should establish assay conditions with small-scale pilot experiments and adhere to institutional SOPs. General (non-product-specific) guidance is provided in the Reaction & Applications and Buffer Applications sections.

Biological Roles

This section describes the biology of the target, beta‑casein, to contextualize research applications. It is general literature information and not product‑specific validation.

• Beta‑casein overview (literature/general)

  • Beta‑casein is a major phosphoprotein component of mammalian milk, belonging to the casein family (αs1, αs2, β, κ). It contributes to casein micelle formation and calcium/phosphate transport.
  • Rich in proline residues, it adopts a flexible, intrinsically disordered structure in solution, enabling diverse interactions.
  • Phosphorylation sites modulate micelle assembly and interactions with minerals; variants (e.g., A1/A2) differ by single amino acid substitutions with biochemical consequences.

• Biological context

  • Synthesized in mammary epithelial cells and secreted into milk; expression is hormonally regulated (prolactin, glucocorticoids).
  • Proteolysis yields bioactive peptides with roles in mineral binding and potential signaling in vitro; relevance depends on processing conditions.

• Research implications

  • Antibodies to beta‑casein are used to study lactation biology, dairy processing, protein variant distribution, and phosphorylation states.

Note: The above describes the target’s biology. It does not constitute application validation for this specific antibody.

Buffer Applications

For antibody handling and assays, buffer composition is critical. The following is general, literature-based guidance; item-specific formulation is not provided for this product.

• Common buffers

  • PBS, pH 7.2–7.6: broadly used for storage, dilutions, washes.
  • TBS, pH 7.4–8.0: preferred in some immunoassays to reduce background.
  • Blocking buffers: PBS/TBS with 1–5% BSA, milk, or serum (note: milk contains caseins and is unsuitable when detecting beta‑casein).

• Preservatives and stabilizers

  • Sodium azide (0.02–0.05%) for storage of antibodies not used on live cells; avoid if peroxidase-based detection is used with certain substrates.
  • Glycerol (10–50%) to permit −20°C storage without freezing; reduces freeze–thaw damage.
  • Protease inhibitors or EDTA when proteolysis is a concern and compatible with downstream assays.

• Wash buffers

  • PBS/TBS with 0.05% Tween‑20 can lower nonspecific binding in ELISA/WB/IHC.

• Special note for anti‑beta‑casein assays

  • Avoid milk as a blocking agent when probing for caseins; use BSA or alternative blockers to prevent competitive inhibition and high background.

• Preparation tips

  • Use high-purity water, filter (0.22 µm), and maintain pH/ionic strength consistency between samples and standards.

Green Alternatives

• Sustainability aspects (general)

  • Recombinant antibodies reduce reliance on animal immunization and hybridoma maintenance, aligning with 3Rs (Replacement, Reduction, Refinement) principles.
  • Consistent sequence-defined production can reduce experimental repetition and waste.

• Packaging and cold chain

  • Shipped on ice packs (from Product Data). Consolidating shipments and choosing local storage reduces transport footprint. Reuse or recycle cold‑chain materials where facilities exist.

• Buffer choices (greener lab practices; literature/general)

  • Use PBS/TBS made from concentrated stocks to minimize packaging waste.
  • Prefer azide-free preservatives when compatible with downstream biological assays to reduce hazardous waste; consider sterile filtration and low-temperature storage instead.
  • Avoid unnecessary single-use plastics by aliquoting into recyclable or reusable formats when biosafety allows.

• Comparative view (general)

  • Animal-derived polyclonals: higher biological resource use and batch variability.
  • Recombinant antibodies: improved consistency and reduced animal use; require cold storage.

• End-of-life and waste

  • Neutral, nonhazardous aqueous buffers can typically enter aqueous waste streams per institutional policy; solutions containing azide or other preservatives may require hazardous waste disposal. Always follow local EHS guidance.

These are general sustainability recommendations; no change to the item’s specifications is implied.

Pharmaceutical Uses

This product is designated For research use only and is not intended for human or veterinary use, diagnostic procedures, or as a therapeutic.

• Research/manufacturing context (general)

  • Antibodies to beta‑casein can support analytical quality control in food and bioprocess laboratories, including detection and quantification of milk proteins in complex matrices (e.g., allergen monitoring, process verification). These are laboratory assay roles, not clinical uses.
  • In preclinical method development, such antibodies may be incorporated into ELISA or lateral-flow assay prototypes for matrix characterization.

• Excipient/formulation status

  • No pharmacopeial excipient role is applicable. There is no monograph-based pharmaceutical use for anti‑beta‑casein antibodies.

• Compliance and documentation

  • For regulated environments, obtain CoA, SDS, and any available validation summaries. Establish in-house method validation (specificity, linearity, precision) if the antibody is used in GMP/GLP workflows.

No therapeutic or diagnostic claims are made or implied for this item.

Physical Properties

Item-specific physicochemical constants are not provided for this antibody.

• Item-specific data

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Concentration/formulation: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

• Literature/general values for IgG-class antibodies (for context, not specifications)

  • Approximate MW: ~150 kDa for full-length IgG; fragments (Fab ~50 kDa, scFv ~25–30 kDa) vary.
  • pI: commonly ~6–9 depending on sequence and glycosylation.
  • Solubility: typically soluble in aqueous buffers (PBS, TBS); stability enhanced with proteins (e.g., 0.1–1% BSA), sugars, or polyols (e.g., 10–50% glycerol) when compatible with application.
  • Stability: freeze–thaw cycles can reduce binding activity; storage at ≤−20°C or 4°C short term depending on formulation.
  • Denaturation temperature: variable; IgG often unfolds above ~60–70°C; functional binding can be lost at lower temperatures if buffer is suboptimal.

• Practical notes (general)

  • Avoid strong oxidants, extremes of pH, and organic solvents which can denature proteins.
  • Gentle mixing is preferable; avoid foaming and vigorous vortexing that may induce aggregation.

All numeric values above are literature/general and not specifications for this item.

Quality and Grades

• Item-specific quality statements (from Product Data)

  • Recombinant: Produced using recombinant expression, enabling consistent lot-to-lot performance versus animal-derived antibodies.
  • ExactAb™: Platform/brand designation indicating engineered, sequence-defined antibody production and quality control. Specific platform details are not provided for this item; refer to CoA/Spec Sheet.
  • Validated: Stated as validated; however, specific tested applications, species reactivity, or performance metrics are not listed for this item.

• Practical implications (general)

  • Recombinant antibodies typically offer high batch consistency, defined sequence, and reduced variability. They are compatible with many immunoassays when formulated appropriately.
  • Validation normally includes functional testing (e.g., binding to target in one or more applications). Without item-specific details, users should perform in-house qualification under their conditions.

• What is not specified for this item

  • Isotype, clonality, host species, epitope/affinity, concentration, purity method (e.g., Protein A/G), stabilizers/preservatives, and endotoxin level are not specified; consult CoA/Spec Sheet.

• Recommendations

  • Request CoA and lot-specific data before critical experiments.
  • Perform small-scale pilot tests to confirm performance in the intended matrix and assay format.

Reaction and Applications

Chemical reaction applications are not applicable to antibody reagents. Instead, consider immunochemical and analytical applications.

• Not specified for this item (from Product Data)

  • Tested applications, recommended dilutions, species cross-reactivity, and positive controls are not provided; refer to CoA/Spec Sheet.

• Typical research uses (literature/general for anti–beta-casein antibodies)

  • Western blotting (WB) to detect beta‑casein in dairy protein extracts or cell/organism models expressing caseins.
  • ELISA for quantifying beta‑casein content or monitoring processing in dairy science and food analysis.
  • Immunoprecipitation (IP) for enrichment of beta‑casein complexes to study interactions or post‑translational modifications (e.g., phosphorylation).
  • Immunohistochemistry/immunofluorescence (IHC/IF) in mammary tissue models, where applicable.

• Practical tips (general)

  • Validate antibody performance against a positive control (e.g., purified beta‑casein standard) and a relevant negative control.
  • Titrate antibody concentration to minimize background while preserving signal; optimize blocking and wash conditions.
  • When assessing phosphorylated vs non‑phosphorylated beta‑casein, ensure the antibody’s epitope compatibility; this item’s epitope specificity is not specified.

Use cases above are general literature guidance and not validated claims for this specific item.

Reaction Conditions

Classical chemical reaction conditions are not applicable to antibody reagents. For experimental use, consider general immunoassay incubation parameters (literature/general), noting that none are specified or validated for this item:

• Incubation temperatures: commonly room temperature or 4°C to preserve binding.
• Incubation times: primary antibody incubations often range from 1 h at room temperature to overnight at 4°C, depending on assay and affinity.
• Buffers: PBS/TBS with appropriate blockers and detergents; compositions should be optimized per assay.
• Sample preparation: denaturing (SDS‑PAGE/WB) vs native conditions (ELISA/IP) affect epitope availability; choose accordingly.

These parameters are general guidance only and are not item-specific conditions. Refer to your internal SOPs and optimize empirically.

Safety and Handling

• Regulatory/safety designation

  • Research Use: For research use only (from Product Data); not for diagnostic or therapeutic use.
  • GHS/CLP: Not specified for this item; refer to the SDS for authoritative classification.

• Hazards and precautions (general for protein antibodies)

  • Typically considered low hazard; however, as a protein solution/lyophilizate, treat as a laboratory chemical.
  • Potential risks include microbial contamination or trace preservatives (if present; not specified) that may cause irritation.

• PPE and hygiene

  • Recommended: lab coat, nitrile gloves, and safety glasses. Wash hands after handling. Avoid ingestion or inhalation of aerosols.

• Handling practices

  • Minimize freeze–thaw by aliquoting upon first thaw.
  • Keep on ice during use to preserve activity. Do not heat unless protocol requires and stability is confirmed.
  • Avoid vigorous agitation or foaming to reduce aggregation.

• Incompatibilities (general)

  • Strong acids/bases, oxidizers, proteases, and many organic solvents can denature proteins.

• First aid (general guidance; defer to SDS)

  • Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing; seek medical advice if irritation persists.
  • Inhalation: Move to fresh air if aerosol exposure suspected.
  • Ingestion: Rinse mouth; seek medical advice.

Always consult the product-specific SDS for definitive hazard and response information.

Solvent Selection

This product is an aqueous protein reagent; selection focuses on buffer systems rather than organic solvents.

• Applicability

  • Organic solvent selection (e.g., for synthetic chemistry) is not typically relevant. See Buffer Applications for practical buffer guidance.

• General buffer suitability (literature/general)

  • Common working/storage buffers: PBS (pH 7.2–7.6) or TBS (pH 7.4–8.0) with optional stabilizers such as 0.02–0.05% sodium azide (not for live cells), 0.1–1% BSA, 10–50% glycerol for −20°C storage.
  • Avoid high concentrations of DMSO, acetone, acetonitrile, or alcohols, which can denature antibodies.

• Additive considerations

  • Detergents: low levels of mild nonionic detergents (e.g., 0.05% Tween‑20) can reduce nonspecific binding in assays but may interfere in some applications.
  • Chelators: EDTA may be used to inhibit metalloproteases when compatible with downstream assays.

• Compatibility notes

  • For ELISA/WB/IHC, buffer composition can markedly affect binding and background. Optimize salt, pH, and blocking proteins to match the sample matrix and target antigen.

All details above are general guidance; item-specific formulation is not specified for this product.

Storage and Reconstitution

• Item-specific storage/shipping (from Product Data)

  • Storage Conditions: Store at −20°C. Avoid repeated freezing and thawing.
  • Shipped In: Ice chest + ice pads (cold pack).

• Practical storage guidance (general)

  • Aliquot upon first thaw to minimize freeze–thaw cycles; use low‑protein binding tubes.
  • If formulation permits, add sterile glycerol (e.g., 40–50%) to store at −20°C without freezing; verify compatibility with your application.
  • For short-term use (≤1–2 weeks), storage at 2–8°C may be suitable if preservative is present and sterility is maintained.
  • Protect from microbial contamination; practice aseptic technique when opening and aliquoting.

• Reconstitution

  • Form (liquid vs lyophilized) and reconstitution buffer/volume are not specified for this item; refer to CoA/Spec Sheet. If lyophilized, typical practice is to reconstitute with sterile buffer (e.g., PBS) to a convenient stock concentration and allow gentle mixing without vortexing.

• Handling

  • Thaw on ice; mix by gentle inversion. Avoid foaming.
  • Do not heat; prolonged exposure to ambient temperatures can reduce activity.

Follow the SDS and CoA for any lot-specific instructions.

Structure and Identity

This product is a recombinant antibody directed against beta-casein (milk casein protein). As a biologic macromolecule, it does not have a single small‑molecule structure.

• Item-specific identifiers

  • Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES / InChI / InChIKey: Not applicable for protein antibodies; not specified for this item.
  • Grade/Purity (from Product Data): Recombinant, ExactAb™, Validated.

• Structural features (literature/general for IgG-type antibodies)

  • Typical architecture: two heavy chains and two light chains forming a Y‑shaped immunoglobulin with variable antigen‑binding domains (Fabs) and an Fc region.
  • Disulfide bonds stabilize inter- and intra-chain domains; each domain adopts an immunoglobulin beta‑sandwich fold.
  • Binding: paratope recognizes an epitope on beta‑casein (linear or conformational; epitope details not specified for this item).

• Form/format

  • Exact isotype, clonality (mono/polyclonal), species origin, concentration, and buffer are not specified for this item; refer to CoA/Spec Sheet.

• 2D/sequence description (literature/general)

  • Antibodies are composed of variable (V) and constant (C) regions; variability in CDR loops of VH/VL determines specificity for beta‑casein. Sequence information is not provided for this item.

Synthetic Utility

Not applicable. As a recombinant antibody reagent, this product is not used as a building block or reagent in chemical synthesis. For practical information relevant to this item, see Buffer Applications, Reaction & Applications, and Storage & Reconstitution.

Target Specificity

Information in this section is limited to product data provided.

• Antigen/Target: beta Casein (as stated in the Product Name).
• Antibody type: Recombinant (from Product Data: Grade/Purity).
• Platform/Brand: ExactAb™ (from Product Data).

Not specified for this item; refer to CoA/Spec Sheet for:

• Clone ID, isotype, host species, light chain type.
• Epitope mapping (linear vs conformational), affinity/avidity.
• Cross-reactivity or species reactivity profile.
• Tested applications and positive controls.

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant beta Casein Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.