GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Bid Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Specifications
Product Name
Recombinant Bid Antibody - Primary antibody, specific to BID, Rabbit IgG
Synonyms
apoptic death agonist | BH3 interacting domain death agonist | BH3-interacting domain death agonist | BID isoform ES(1b) | BID isoform L(2) | BID isoform Si6 | BID | desmocollin type 4 | FP497 | Human BID coding sequence | MGC15319 | MGC42355 | p22 BID |
Specifications & Purity
Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms
The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Host species
Rabbit
Specificity
BID
Immunogen
A synthetic peptide derived from human BID (AA 30-86)
Positive Control
WB: Jurkat, MOLT-4 and HEK293 cell lysates. IHC: Human lung tissue. IF/ICC: Jurkat cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Bid Antibody (Ab091243) - Western Blot All lanes: Recombinant Bid Antibody (Ab091243) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 10.8 s
Recombinant Bid Antibody (Ab091243) - IF/ICC Immunocytochemistry analysis of Bid (green) in Jurkat using Recombinant Bid Antibody (Ab091243) and DAPI (blue).
Recombinant Bid Antibody (Ab091243) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung tissue using Recombinant Bid Antibody (Ab091243). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
IF/ICC
1/50 - 1/200
WB
1/1000-1/2000
IHC
1/100-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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