Recombinant Brachyury/Bry mAb - Anticorpo primario, specific to Brachyury/Bry, Rabbit IgG

Cat. No.: Ab091590

GRADE & PURITY Recombinant, ExactAb™, Validated, See COA

Application

WB

Host species

Coniglio

Reactive species

consiglio, Mouse, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human Brachyury/Bry Antibody, Recombinant, could be used for WB and so on.

Specifications

Product Name

Recombinant Brachyury/Bry mAb - Anticorpo primario, specific to Brachyury/Bry, Rabbit IgG

Sinonimi

Brachyury | MGC104817 | Protein T | SAVA | T brachyury (mouse) homolog | T brachyury homolog | T Brachyury Transcription Factor | T, brachyury homolog (mouse) | T-Box Transcription Factor T | TBXT | TF | TFT | BRAC_HUMAN antibody | brachyury protein | Bra

Specifiche e purezza

Recombinant, ExactAb™, Validated, See COA

Meccanismi biochimici e fisiologici

Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.

Specie ospite

Coniglio

Specificità

Brachyury/Bry

Immunogeno

A synthetic peptide derived from human Protein T (AA 250-435)

Controllo positivo

WB: MUG-Chor1, C6 lysates.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant Brachyury Antibody (Ab091590)

Recombinant Brachyury Antibody (Ab091590) - Western Blot
All lanes: Recombinant Brachyury Antibody (Ab091590) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 47 kDa
Observed band size: 38 kDa
Exposure time: 150.0 s

Western Blot: Recombinant Brachyury Antibody (Ab091590)

Recombinant Brachyury Antibody (Ab091590) - Western Blot
Western blot analysis of Brachyury expression in MUG-Chor1 (human sacral bone chordoma) cell lysate.

Predicted band size: 47 kDa
Observed band size: 41, 47 kDa

Applicazione

Applicazione

Dilution info

WB

1/500-1/2000

Obiettivi associati (umani)

TBXT Tbio T-box transcription factor T (0 Activities)

Discover Target: TBXT

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Item-specific facts (from Product Data)

• Manufacturer-tested applications, recommended dilutions, antigen retrieval methods, and positive control materials for Recombinant Brachyury/Bry mAb (Ab091590) are not specified for this item; refer to the CoA/Spec Sheet.

Note

• As required by the product data policy, only manufacturer-provided protocols and application validations can be listed here. Please consult the item’s documentation for detailed, validated procedures.

Biological Roles

Target overview (literature)

• Brachyury (Bry, T) is a T-box family transcription factor central to mesoderm specification, notochord formation, and early gastrulation in vertebrate embryogenesis.
• It regulates genes involved in cell movement and differentiation during early development. Expression is tightly timed/spatially restricted and is a canonical marker of mesodermal lineage commitment in pluripotent stem cell differentiation protocols.

Research relevance

• Brachyury expression serves as a readout for directed differentiation of human ESCs/iPSCs toward mesodermal fates. It is also used to map developmental trajectories and construct gene regulatory networks in early embryos across species.
• In adult/pathological contexts, altered Brachyury expression has been observed in certain tissues in the literature; researchers investigate its roles in epithelial–mesenchymal transitions and lineage plasticity. No clinical or therapeutic claims are made here.

Antibody utility

• An anti-Brachyury mAb enables selective detection of nuclear Brachyury protein in fixed cells/tissues or cell lysates, supporting temporal and spatial expression profiling.

Item-specific note

• Epitope region, isotype, species reactivity, and cross-reactivity for Recombinant Brachyury/Bry mAb (Ab091590) are not specified for this item; refer to the CoA/Spec Sheet for target-binding particulars and validated species.

Buffer Applications

Applicability note

• This is an antibody reagent; it is used within biological buffer systems rather than acting as a buffering agent itself.

General guidance (literature)

• Common working buffers: PBS or TBS at pH ~7.2–7.6. Additives such as 0.05% Tween-20 can reduce nonspecific binding in wash steps.
• Blocking solutions: 5% serum (species-appropriate), BSA (1–5%), or casein-based blockers to minimize background.
• For IHC, antigen retrieval buffers (e.g., citrate pH ~6, Tris-EDTA pH ~9) may be used depending on fixation and tissue; optimize empirically for nuclear antigens like Brachyury.
• For WB, transfer and blocking buffers should be optimized to preserve epitope integrity; inclusion of mild detergents can aid specificity.

Item-specific note

• The supplied storage buffer, recommended working dilution buffer, and compatibility specifics for SKU Ab091590 are not specified for this item; please consult the CoA/Spec Sheet.

Green Alternatives

Applicability note

• “Green alternatives” in the context of an antibody product primarily relate to buffer and workflow choices rather than solvent substitution for chemical reactions.

Greener practice suggestions (general)

• Prefer phosphate-buffered saline (PBS) or TBS with minimal hazardous preservatives when compatible with your assay and biosafety policies.
• Reduce single-use plastics by employing reusable, validated containers where contamination risk is controlled; use low-retention tips/tubes efficiently to minimize waste.
• Choose non-toxic blocking agents and detergents where possible; avoid high concentrations of azide in workflows that lead to hazardous waste where institutional rules discourage it.
• Optimize antibody titers to reduce reagent consumption without compromising data quality.

Trade-offs

• Lower preservative concentrations may reduce shelf life or microbial robustness; balance safety goals with product stability.

Item-specific note

• The precise formulation and preservative profile for SKU Ab091590 are not specified for this item; consult the CoA/Spec Sheet to plan greener buffer alternatives that maintain stability.

Pharmaceutical Uses

Applicability note

• This reagent is for research use only and is not intended for diagnostic or therapeutic applications.

Context (general)

• Monoclonal antibodies can serve as analytical tools in bioprocess development and quality control (e.g., ligand-binding assays, impurity tracking) but such uses require application-specific validation.
• No pharmacopeial monograph, dosage form, or excipient role is provided for this product.

Item-specific statement

• There are no pharmaceutical/formulation uses specified for Recombinant Brachyury/Bry mAb (Ab091590); refer to institutional guidelines if considering any regulated application, and contact Aladdin Scientific for documentation relevant to research-only workflows.

Physical Properties

Item-specific facts (from Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration, buffer composition, preservative, and exact protein content: Not specified for this item; refer to CoA/Spec Sheet.

Applicability note

• Conventional physicochemical constants (bp, mp, density, refractive index, logP) are not applicable to macromolecular protein biologics such as monoclonal antibodies.

General properties (literature)

• Approximate size: Immunoglobulin G (IgG) antibodies are typically ~150 kDa; hydrodynamic radius ~5–6 nm; isoelectric point can vary (commonly pI 6–9 depending on isotype and sequence).
• Solubility: Readily soluble in aqueous physiological buffers (e.g., PBS, TBS) with stabilizers (e.g., 0.1–1% carrier protein or polyols) where specified by supplier; avoid extreme pH and high ionic detergents unless validated for the application.
• Stability: Sensitive to repeated freeze–thaw and prolonged exposure to elevated temperature; stability is strongly formulation-dependent.

Practical implications

• Handle on ice where feasible; keep in recommended buffer; avoid vigorous vortexing/foaming to minimize aggregation.
• For exact formulation, concentration, and excipients for SKU Ab091590, consult the item’s CoA/Spec Sheet.

Quality and Grades

Item-specific facts (from Product Data)

• Grade/Purity: Recombinant, ExactAb™, Validated

What these designations mean (general)

• Recombinant mAb: Sequence-defined production in a controlled host system (e.g., mammalian or other expression), providing enhanced lot-to-lot consistency compared with hybridoma-derived monoclonals.
• ExactAb™: Platform designation indicating precise, recombinant, sequence-verified antibody production with stringent release criteria (general description; see product documentation for platform specifics). The advantage is minimized batch variability and improved reproducibility across experiments.
• Validated: Indicates performance verification in defined applications and/or sample types by the manufacturer. For this specific SKU, the exact validated applications, species reactivity, and sample prep methods are not listed here; refer to the CoA/Spec Sheet for validated use-cases.

Release testing (typical for recombinant mAbs; general)

• Identity/sequence confirmation, concentration determination, purity by SDS-PAGE/SEC, and functional binding assessments (e.g., ELISA/biolayer interferometry) are common. Exact test panel for Ab091590 is not specified for this item; refer to CoA/Spec Sheet.

Practical impact

• Expect strong reproducibility and reduced need for re-optimization across lots; still verify titer and background in your own matrix due to laboratory- and protocol-specific variables.

Reaction and Applications

Applicability note

• As a recombinant monoclonal antibody, this product is used in immunoassay and detection workflows rather than as a reagent in synthetic chemical reactions.

General application domains for anti-Brachyury antibodies (literature)

• Immunodetection of Brachyury (T/Bry), a T-box transcription factor critical for mesoderm formation, in developmental biology, stem cell differentiation models, and embryoid body studies.
• Techniques where anti-Brachyury mAbs are commonly employed include immunofluorescence (IF/ICC) for nuclear localization, immunohistochemistry (IHC) in tissue sections, Western blotting (WB) for size-specific detection, ELISA for quantitative analyses, and flow cytometry (FC) if the epitope is accessible (e.g., after permeabilization).

Practical considerations (general)

• For nuclear transcription factors like Brachyury, antigen retrieval and permeabilization are often required (optimize fixative, retrieval buffer, and detergent).
• Include appropriate positive controls (Brachyury-expressing cells/tissues) and negative controls (knockdown/knockout, isotype control).
• Titrate the antibody to balance signal and background; blocking reagents (serum or protein-based blockers) and optimized wash conditions can improve S/N.

Item-specific note

• Manufacturer-tested applications and dilutions for SKU Ab091590 are not specified here; refer to the CoA/Spec Sheet for validated applications and recommended conditions.

Reaction Conditions

Applicability note

• Conventional reaction conditions (solvent, temperature, catalysts, yields) are not applicable to antibody-based detection reagents.

General method conditions (literature) for immunoassays

• Incubations typically occur at 4–25°C for 1–16 h depending on assay (WB/IHC/IF/ELISA), followed by washes in buffered saline with mild detergent.
• Fixation/permeabilization for nuclear targets like Brachyury commonly uses paraformaldehyde (PFA) and Triton X-100/saponin, or alcohol-based methods depending on downstream needs.
• Signal development uses enzyme substrates or fluorophores under light-protected conditions; blocking and stringent washes reduce background.

Item-specific note

• Exact, validated incubation times, temperatures, and dilutions for Ab091590 are not specified for this item; refer to the CoA/Spec Sheet for manufacturer-validated conditions.

Safety and Handling

Item-specific facts (from Product Data)

• GHS classification, signal word, hazard statements, pictograms: Not specified for this item; refer to the product SDS.
• Research use note: For research use only.

General safety guidance (literature/standard lab practice)

• Personal protective equipment: Lab coat, gloves, and safety glasses when handling antibody solutions and ancillary reagents (fixatives, substrates, secondary antibodies).
• Avoid ingestion, inhalation of aerosols, and contact with skin/eyes. Handle in a clean area to prevent biological contamination of the reagent.
• Incompatibilities: Avoid mixing with strong oxidizers or proteases; avoid exposure to extreme pH (<5 or >9) unless the protocol requires it and stability is validated.
• First aid (overview; defer to SDS): Rinse skin/eyes with water upon contact; seek medical attention if irritation persists. If inhaled as aerosol, move to fresh air; if ingested, rinse mouth and follow institutional procedures.
• Waste: Dispose according to institutional biological and chemical waste guidelines; decontaminate surfaces with appropriate disinfectants.

Notes

• Always consult the SDS for SKU Ab091590 for authoritative hazard, PPE, and spill/first-aid instructions.
• Some antibody formulations may contain preservatives (e.g., sodium azide); check the CoA/SDS for specific handling and disposal considerations.

Solvent Selection

Applicability note

• This product is a recombinant monoclonal antibody intended for aqueous biological workflows. Traditional “solvent selection” (organic solvent choice by polarity, dielectric constant) is not typically applicable.

Practical guidance (general)

• Use isotonic, neutral pH buffers (e.g., PBS or TBS) as recommended by the product documentation.
• Avoid high percentages of organic solvents (e.g., >10–20% methanol, acetonitrile, DMSO) which can denature or precipitate antibodies unless a validated protocol explicitly requires them.
• Low concentrations of gentle nonionic detergents (e.g., 0.05% Tween-20) may be used in wash buffers to reduce nonspecific binding, subject to application compatibility.

Item-specific buffer recommendation

• Exact formulation and compatible buffer systems for SKU Ab091590 are not specified for this item; consult the CoA/Spec Sheet.

Storage and Reconstitution

Item-specific facts (from Product Data)

• Storage conditions: Store at −20°C. Avoid repeated freezing and thawing.
• Shipping: Ice chest + ice pads
• Reconstitution details, working aliquot guidance, and formulation: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/best practice)

• If provided lyophilized: Reconstitute with sterile, antibody-grade water or buffer as recommended by the datasheet; gently mix by inversion; allow to fully dissolve on ice; avoid foaming.
• If provided in solution: Prepare single-use aliquots on receipt to prevent freeze–thaw cycles; store at the recommended temperature. Keep on ice during handling.
• For short-term use (days–weeks), many antibodies are stable at 2–8°C; however, follow the item-specific recommendation of −20°C for Ab091590 to maximize stability and avoid deviations from validated storage.
• Protect from microbial contamination; use sterile technique. Do not use after signs of precipitation or turbidity appear unless validated by test spin/clarification.

Reminder

• For exact buffer composition, concentration, and any stabilizers/preservatives for SKU Ab091590, consult the CoA/Spec Sheet and the SDS.

Structure and Identity

Item-specific facts (from Product Data)

• Product name: Recombinant Brachyury/Bry mAb (SKU: Ab091590)
• Type: Monoclonal antibody (recombinant); platform: ExactAb™; status: Validated
• CAS / CID / InChIKey / SMILES: Not applicable to antibodies; not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula and molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General description (literature)

• Monoclonal antibodies are large glycoproteins (~150 kDa for IgG isotypes) composed of two heavy and two light chains with disulfide linkages; they recognize a single epitope with high affinity/specificity.
• “Recombinant” indicates sequence-defined production in an expression host for lot-to-lot consistency.
• Brachyury (also known as Bry or T) is a T-box transcription factor; antibodies targeting Brachyury are typically used to detect nuclear protein in developmental biology and stem cell differentiation studies.

2D/structural features (descriptive)

• Typical IgG architecture: two Fab antigen-binding arms (variable domains containing CDRs) and one Fc domain (constant region mediating effector interactions). Epitope binding occurs via paratope residues in VH/VL variable regions.

Notes on identity verification (general best practice)

• Verify identity by application-specific performance (e.g., a nuclear signal in Brachyury-positive controls) and sequence documentation provided for recombinant antibodies (if available).

Synthetic Utility

Applicability note

• As a biological detection reagent, this recombinant monoclonal antibody is not used as a substrate, catalyst, or reagent in synthetic organic/inorganic chemistry.

Where it can intersect with chemistry (general)

• Antibodies may be chemically conjugated to enzymes (e.g., HRP, AP), fluorophores, haptens, or solid supports for assay development. Such conjugations typically proceed through amine-reactive (NHS-ester), thiol-reactive (maleimide) chemistries, or click strategies (azide–alkyne) after suitable derivatization.

Item-specific note

• Conjugation-ready format, buffer amine content, and recommended coupling chemistries for SKU Ab091590 are not specified for this item; consult the CoA/Spec Sheet before performing chemical modifications.

Target Specificity

Item-specific facts (from Product Data)

• Antigen/target: Brachyury/Bry (T-box transcription factor)
• Clone, isotype, host species, species reactivity, epitope, and cross-reactivity: Not specified for this item; refer to CoA/Spec Sheet.

General notes (literature)

• Brachyury is a nuclear protein; high-quality mAbs typically recognize a defined linear or conformational epitope within the T-box or adjacent regions. Nuclear localization necessitates appropriate fixation/permeabilization for detection.

Verification practices (general)

• Validate specificity using knockout/knockdown controls, peptide competition (if epitope peptide is available), and orthogonal methods (qPCR of T gene or proteomics) where feasible.