Recombinant Caspase3 Antibody - Anticorpo primario, specific to CASP3, Rabbit IgG

Cat. No.: Ab093013

GRADE & PURITY Recombinant, ExactAb™, Validated, 0.9mg/mL

Application

IF/ICC, IHC, WB

Host species

Coniglio

Reactive species

Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human Caspase3 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

Specifications

Product Name

Recombinant Caspase3 Antibody - Anticorpo primario, specific to CASP3, Rabbit IgG

Sinonimi

A830040C14Rik antibody | Apopain antibody | CASP 3 antibody | CASP-3 antibody | CASP3 antibody | CASP3_HUMAN antibody | Casp3a antibody | Caspase 3 antibody | Caspase 3, apoptosis-related cysteine peptidase antibody | Caspase 3, apoptosis-related cysteine

Specifiche e purezza

ExactAb™, Validated, Recombinant, 0.9mg/mL

Specie ospite

Coniglio

Specificità

CASP3

Immunogeno

A synthetic peptide derived from human Caspase-3 (AA 75-94).

Controllo positivo

WB: Jurkat, HeLa,THP-1,Hep G2 and K562 cell lysates; IHC: Human bladder cancer tissues; IF: 293

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Immunogen affinity purified

Concentrazione

0.9mg/mL

Nota

This product is about to be sold out and discontinued. The replacement product is Ab093009

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant Caspase3 Antibody (Ab093013)

Recombinant Caspase3 Antibody (Ab093013) - Western Blot
All lanes: Recombinant Caspase3 Antibody (Ab093013) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 32 kDa
Observed band size: 30 kDa
Exposure time: 1.0 s

ICC/IF: Recombinant Caspase3 Antibody (Ab093013)

Recombinant Caspase3 Antibody (Ab093013) - ICC/IF
IF analysis of HEK293 cells using Recombinant Caspase3 Antibody (Ab093013) (green),and cells were counterstained with DAPI (blue).

IHC: Recombinant Caspase3 Antibody (Ab093013)

Recombinant Caspase3 Antibody (Ab093013) - IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using Recombinant Caspase3 Antibody (Ab093013). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Applicazione

Applicazione

Dilution info

WB

1/500-1/1000

IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Obiettivi associati (umani)

CASP3 Tchem Caspase-3 (0 Activities)

Discover Target: CASP3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CASP3 Tchem Caspase-3 (3632 Activities)

Discover Target: CASP3

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Caspase3 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.