Recombinant Caveolin-2 Antibody - Anticorpo primario, specific to CAV2, Rabbit IgG

Cat. No.: Ab093252

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.2 mg/mL

Application

IF/ICC, IHC, WB

Host species

Coniglio

Reactive species

Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human Caveolin-2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500 - 1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
Post-translational
Post-translational': "Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.

Specifications

Product Name

Recombinant Caveolin-2 Antibody - Anticorpo primario, specific to CAV2, Rabbit IgG

Sinonimi

CAV antibody | CAV2 antibody | CAV2_HUMAN antibody | Caveolae protein 20 kD antibody | Caveolin 2 antibody | Caveolin 2 isoform a and b antibody | Caveolin-2 antibody | MGC12294 antibody | OTTHUMP00000025032 antibody | OTTHUMP00000195982 antibody | CAV |

Specifiche e purezza

ExactAb™, Validated, Recombinant, 0.2 mg/mL

Specie ospite

Coniglio

Specificità

CAV2

Immunogeno

A synthetic peptide derived from human Caveolin-2 (AA 14-27)

Controllo positivo

WB: HeLa, A549, SK-OV-3, U2OS and HUVEC cell lysates. IHC: Human lung cancer tissue. ICC/IF: A549 cells.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Antigen affinity purified

Concentrazione

0.2 mg/mL

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant Caveolin-2 Antibody (Ab093252)

Recombinant Caveolin-2 Antibody (Ab093252) - Western Blot
All lanes: Recombinant Caveolin-2 Antibody (Ab093252) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 18 kDa
Observed band size: 18, 20 kDa
Exposure time: 23.1 s

IF/ICC: Recombinant Caveolin-2 Antibody (Ab093252)

Recombinant Caveolin-2 Antibody (Ab093252) - IF/ICC
Immunocytochemistry analysis of Caveolin 2 (green) in A549 using Recombinant Caveolin-2 Antibody (Ab093252), and cells were counterstained with DAPI (blue).

IHC: Recombinant Caveolin-2 Antibody (Ab093252)

Recombinant Caveolin-2 Antibody (Ab093252) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant Caveolin-2 Antibody (Ab093252). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Applicazione

• Applicazione: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

• IHC:

  1/50 - 1/100;

Obiettivi associati (umani)

CAV2 Tbio Caveolin-2 (0 Activities)

Discover Target: CAV2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Caveolin-2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.