Recombinant CD45 Antibody - Anticorpo primario, specific to CD45

Cat. No.: Ab210608

GRADE & PURITY Animal Free, Carrier Free, Recombinant, Validated, ExactAb™, Azide Free, High Performance, ≥95%(SDS-PAGE), See COA

Application

Flow Cytometry

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG1

Clone Number

P51B13

Conjugation

Unconjugated

Panoramica

Recombinant CD45 Antibody, clone P51B13, could be used for Flow Cytometry, ELISA and so on.

Specifications

Product Name

Recombinant CD45 Antibody - Anticorpo primario, specific to CD45

Sinonimi

B220 | CD45 | CD45 antigen | CD45R | EC 3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase | PTPRC | receptor-type tyrosine-protein phosphatase C | T200 Glycoprotein | T200 leukocyte common antigen | T200 | B220

Specifiche e purezza

Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), See COA

Meccanismi biochimici e fisiologici

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second

Specie ospite

Mouse

Specificità

CD45

Immunogeno

Peripheral blood mononuclear cells

Coniugazione

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Recombinant, Validated

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Immagini

Flow Cytometry: Recombinant CD45 Antibody (Ab210608)

Recombinant CD45 Antibody (Ab210608) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 with Recombinant CD45 Antibody (Ab210608) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Mouse IgG (Biotin) (Ab180306) - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: Recombinant CD45 Antibody (Ab210608)

Recombinant CD45 Antibody (Ab210608) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 (red) with Recombinant CD45 Antibody (Ab210608) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab180306) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Recombinant CD45 Antibody (Ab210608)

Recombinant CD45 Antibody (Ab210608) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with Recombinant CD45 Antibody (Ab210608). Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

SEC: Recombinant CD45 Antibody (Ab210608)

Recombinant CD45 Antibody (Ab210608) - SEC
The purity of Recombinant CD45 Antibody (Ab210608) is more than 95% verified by HPLC.

Applicazione

• Applicazione: Dilution info
• Flow Cytometry:

  ≤0.5 µg per million cells in 100 µl volume;

Domande frequenti

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CD45 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.