Recombinant CD45RO Antibody - Anticorpo primario, specific to Human CD45RO

Cat. No.: Ab212153

QUALITÀ & PUREZZA Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), See COA

Application

ELISA, Flow Cytometry

Host species

Mouse

Reactive species

Umano

Isotype

Mouse IgG2a

Clone Number

P08F59

Conjugation

Unconjugated

Panoramica

Recombinant CD45RO Antibody, clone P08F59, could be used for Flow Cytometry, ELISA and so on.

Specifiche

Product Name

Recombinant CD45RO Antibody - Anticorpo primario, specific to Human CD45RO

Sinonimi

B220 | CD45 antigen | CD45R | CD45RO | EC 3.1.3.48 | L-CA | LY5 | protein tyrosine phosphatase, receptor type, C | receptor-type tyrosine-protein phosphatase C | T200 glycoprotein | T200 leukocyte common antigen | T200receptor type, c polypeptide | B220 a

Specifiche e purezza

Animal Free,Carrier Free,Recombinant,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),See COA

Meccanismi biochimici e fisiologici

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second

Specie ospite

Mouse

Specificità

Human CD45RO

Immunogeno

IL-2 dependent T cell line, CA1

Controllo positivo

Flow Cytometry: Human peripheral blood lymphocytes

Coniugazione

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Recombinant, Validated

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Product Line

Prodotti biologici

Stoccaggio e spedizione

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Immagini

Flow Cytometry: Recombinant CD45RO Antibody (Ab212153)

Recombinant CD45RO Antibody (Ab212153) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45RO (red) with Recombinant CD45RO Antibody (Ab212153) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

SEC: Recombinant CD45RO Antibody (Ab212153)

Recombinant CD45RO Antibody (Ab212153) - SEC
The purity of Recombinant CD45RO Antibody (Ab212153) is more than 95% verified by HPLC.

Applicazione

Applicazione

Dilution info

Flow Cytometry

≤0.5 µg per million cells in 100 µl volume

Obiettivi associati (umani)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CD45RO Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.