GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human CD86 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant CD86 Antibody - Primary antibody, specific to CD86, Rabbit IgG
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as d
Host species
Rabbit
Specificity
CD86
Immunogen
A synthetic peptide derived from human CD86 (AA 5-60).
Positive Control
WB: Raji, HeLa, Jurkat, MCF7, SH-SY5Y, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. IF/ICC: HeLa, K562 cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant CD86 Antibody (Ab095458) - Western Blot All lanes: Recombinant CD86 Antibody (Ab095458) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 73, 97 kDa The expression profile and molecular weight observed is mainly due to glycosylation and has been described in the literature (PMID:21896490). Exposure time: 0.9 s
Recombinant CD86 Antibody (Ab095458) - IHC Immunohistochemical analysis of paraffin-embedded human tonsil, using Recombinant CD86 Antibody (Ab095458) .
Recombinant CD86 Antibody (Ab095458) - IF/ICC Immunofluorescent analysis using Recombinant CD86 Antibody (Ab095458) at 1/50 dilution.
Recombinant CD86 Antibody (Ab095458) - IF/ICC Immunohistochemical analysis of paraffin-embedded human tonsil, using Recombinant CD86 Antibody (Ab095458) .
Recombinant CD86 Antibody (Ab095458) - IF/ICC Immunohistochemical analysis of paraffin-embedded human tonsil, using Recombinant CD86 Antibody (Ab095458) .
Recombinant CD86 Antibody (Ab095458) - IF/ICC Immunofluorescent analysis of K562 cells, using Recombinant CD86 Antibody (Ab095458) .
Application
Application
Dilution info
IF/ICC
1/50-1/200
WB
1/1000-1/2000
IHC
1/100-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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