Recombinant Cdc34 Antibody - Anticorpo primario, specific to Cdc34, Rabbit IgG

Cat. No.: Ab095618

GRADE & PURITY Recombinant, ExactAb™, Validated, See COA

Application

IF/ICC, WB

Host species

Coniglio

Reactive species

consiglio, Mouse, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human Cdc34 Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/2000
ICC/IF: 1/50-1/200
Protein Function：
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase throught its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.

Specifications

Product Name

Recombinant Cdc34 Antibody - Anticorpo primario, specific to Cdc34, Rabbit IgG

Sinonimi

Cdc 34 antibody | Cdc34 antibody | Cell division cycle 34 antibody | Cell division cycle 34 homolog (S. cerevisiae) antibody | Cell division cycle 34 homolog antibody | E2 CDC34antibody | UB2R1_HUMAN antibody | UBC 3 antibody | UBC3 antibody | UBCH3 antib

Specifiche e purezza

Recombinant, ExactAb™, Validated, See COA

Specie ospite

Coniglio

Specificità

Cdc34

Immunogeno

A synthetic peptide derived from human Cdc34 (AA 2-236).

Controllo positivo

WB: Jurkat, Hep G2, K562, A549, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant Cdc34 Antibody (Ab095618)

Recombinant Cdc34 Antibody (Ab095618) - Western Blot
All lanes: Recombinant Cdc34 Antibody (Ab095618) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 27 kDa
Observed band size: 31 kDa
Exposure time: 42.7 s

IF/ICC: Recombinant Cdc34 Antibody (Ab095618)

Recombinant Cdc34 Antibody (Ab095618) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Cdc34 Antibody (Ab095618).

IF/ICC: Recombinant Cdc34 Antibody (Ab095618)

Recombinant Cdc34 Antibody (Ab095618) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Cdc34 Antibody (Ab095618) at 1/25 dilution.

IF/ICC: Recombinant Cdc34 Antibody (Ab095618)

Recombinant Cdc34 Antibody (Ab095618) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Cdc34 Antibody (Ab095618) at 1/100 dilution.

Applicazione

Applicazione

Dilution info

WB

1/500-1/2000

IF/ICC

1/50-1/200

Obiettivi associati (umani)

CDC34 Tbio Ubiquitin-conjugating enzyme E2 R1 (0 Activities)

Discover Target: CDC34

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Not specified for this item; refer to CoA/Spec Sheet.

Only item-specific, tested applications (e.g., WB, IHC, IF, FC), recommended dilutions, and controls validated by the manufacturer should be followed when provided.

Biological Roles

Target background (literature; general, not item-specific claims):

• Cdc34 (also known as UBE2R1) is an E2 ubiquitin-conjugating enzyme central to SCF (SKP1–CUL1–F-box) E3 ligase function. It catalyzes ubiquitin transfer from E1 to substrate-bound complexes, regulating proteasomal degradation of key cell-cycle regulators.
• Biological processes: Cell-cycle progression (G1/S transition), DNA replication licensing, and protein quality control via ubiquitin–proteasome system. Cdc34 activity affects the turnover of cyclins and CKIs in various model systems.
• Subcellular localization: Predominantly cytosolic/nuclear depending on cell state and species; association with SCF components influences localization.
• Orthology: Conserved from yeast Cdc34 to mammalian UBE2R1/R2, enabling cross-species studies with appropriate antibodies.

Reagent relevance:

• Antibodies against Cdc34 enable detection/quantitation of protein abundance, assessment of pathway engagement, and immunoprecipitation of complexes for ubiquitination studies.

Item-specific notes: Epitope, species reactivity, clonality, and isotype for Ab095618 are not specified for this item; refer to CoA/Spec Sheet to determine suitability for your system and application.

Buffer Applications

Item-specific buffer composition is not provided; refer to CoA/Spec Sheet. General guidance for antibody handling and assay buffers (literature, not item-specific):

• Storage/dilution buffers: PBS (10–50 mM phosphate, 150 mM NaCl, pH 7.2–7.4) or TBS (20–50 mM Tris, 150 mM NaCl, pH 7.4–7.6). Include 0.01–0.05% Tween-20 for washing/dilution in immunoassays to reduce nonspecific binding.
• Blocking buffers: 3–5% BSA or nonfat dry milk for Western blot; serum or commercial blockers for IHC/IF depending on species and detection system.
• Carrier proteins: 0.1–1% BSA can stabilize dilute antibody solutions and minimize adsorption to plastics; omit carrier for conjugation workflows.
• Antimicrobials: 0.02–0.05% sodium azide for long-term refrigerated storage when compatible with downstream enzymes/cells; avoid azide with HRP conjugates in solution or live-cell applications.
• Antigen retrieval (IHC): Choice of citrate (pH ~6) vs Tris-EDTA (pH ~9) buffers is target-dependent; optimize empirically for Cdc34 detection.

Note: The precise buffer supplied with Ab095618, presence/absence of azide, protein carriers, and recommended dilutions are not specified here. Validate buffer compatibility for your chosen assay and consult the product datasheet for any item-specific recommendations.

Green Alternatives

While “green alternatives” usually concerns solvent substitution, sustainability principles apply to antibody workflows as well. Consider the following practices (general guidance; not item-specific):

• Cold-chain optimization: This product ships cold (ice chest + ice pads). Consolidate orders and select shipping options that minimize transit time and packaging waste while maintaining product integrity.
• Freeze–thaw minimization: Product Data advises avoiding repeated freeze–thaw. Aliquot upon receipt to reduce waste and extend usable life, thereby lowering environmental and cost impact.
• Buffer choices: Use phosphate- or Tris-based buffers prepared from concentrates to reduce packaging. Avoid unnecessary azide when compatible with downstream use (e.g., live-cell applications), or consider ProClin-type biocides at minimal effective concentrations.
• Plastics reduction: Employ reusable glass serological reservoirs where appropriate; choose low-bind, recyclable plastics when single-use is necessary.
• Energy use: Store at the warmest validated temperature that preserves stability (−20 °C per Product Data) rather than defaulting to −80 °C unless data support it.

Comparison (general):

• Glycerol preservation vs repeated freezing: Glycerol-containing storage at −20 °C often allows nonfreezing storage, improving longevity and reducing freeze–thaw cycles; verify compatibility with downstream assays before use.

Note: No change to the product formulation is recommended without verifying performance on your specific application.

Pharmaceutical Uses

For research use only (per Product Data). Not for diagnostic, clinical, or therapeutic use.

Contextual, non-clinical information (general):

• Role in biopharma R&D: Research-grade antibodies to pathway proteins like Cdc34 are commonly used in target validation, mechanism-of-action studies, and development of pharmacodynamic assays in preclinical discovery settings.
• Assay development: Antibodies can be integrated into ELISA or immunoassay platforms for in-process controls, potency assays, and biomarker method development. Carrier-free preparations are preferred for conjugation to enzymes or fluorophores in such assay systems.
• Quality considerations: For regulated environments, additional documentation (traceability, stability, lot release criteria) is often required; this information is not specified for Ab095618 and should be requested as needed.

Item-specific details such as compliance with pharmacopeial standards, endotoxin levels, bioburden, or residual host-cell protein/DNA are not specified for this item; refer to the CoA/Spec Sheet if such attributes are relevant to your application.

Physical Properties

Item-specific physical data: Not specified for this item; refer to CoA/Spec Sheet.

• Appearance: Not specified
• Concentration/formulation: Not specified
• Molecular weight: Not specified (format-dependent for recombinant antibodies)
• pI, extinction coefficient, buffer composition, stabilizers/preservatives: Not specified

General/literature guidance for recombinant antibodies (not item-specific):

• Typical molecular mass: ~150 kDa for full-length IgG; ~50 kDa for Fab; ~25–30 kDa for scFv. Actual value depends on format and glycosylation.
• Solubility: Highly soluble in aqueous isotonic buffers (e.g., PBS, TBS) with protein carriers (e.g., BSA) to minimize adsorption.
• Stability considerations: Sensitive to repeated freeze–thaw; stability enhanced by glycerol (typically 30–50% v/v) and storage at −20 °C or below. Avoid vigorous agitation and elevated temperatures.
• pH range for stability: Many IgG formats are stable near neutral pH (6.5–7.5), though this is antibody- and formulation-dependent.

Note: The above are literature norms for antibody reagents and are not specifications for SKU Ab095618. Consult the product CoA/Spec Sheet for exact composition and measured properties.

Quality and Grades

Item-specific quality designations (from Product Data):

• Recombinant
• ExactAb™
• Validated

What these mean in practice (general explanations; not new specs):

• Recombinant: Sequence-defined production in a controlled expression system (e.g., mammalian or other host), providing lot-to-lot consistency, reduced background from serum proteins, and the potential for engineered formats/isotypes. Recombinant manufacture typically allows better control over glycosylation and eliminates hybridoma drift.
• ExactAb™: Proprietary Aladdin Scientific designation highlighting standardized production and internal QC workflows aimed at reproducibility. The specific QC tests and acceptance criteria for this item are not specified; refer to CoA/Spec Sheet.
• Validated: Indicates this clone/preparation has undergone application-specific or target-specific verification. The exact applications, sample types, and validation datasets for SKU Ab095618 are not specified here; consult the datasheet/CoA for supported uses and performance characteristics.

Additional notes:

• Purity format (e.g., Protein A/G affinity, SEC polish), buffer composition, and preservatives are not specified for this item. If low background/low UV is important (e.g., in assay development or analytical detection), review the CoA for UV–Vis profiles, residual host-cell proteins/DNA, and endotoxin if relevant to your workflow.
• For regulated workflows, request documentation such as CoA, lot traceability, and application validation summaries for the specific lot you receive.

Reaction and Applications

This section is typically oriented to small-molecule reaction chemistry and does not directly apply to antibody reagents. For recombinant antibodies like SKU Ab095618, the relevant use cases are analytical and detection assays (e.g., immunoassays) rather than chemical transformations.

• Not applicable: Named organic reactions, catalysts, and synthetic mechanisms do not pertain to this product.
• For practical use: See Target Specificity and Application Protocols tabs for antibody-related details. For this item, those specifics are not provided and should be obtained from the CoA/Spec Sheet.

General guidance (literature, not item-specific):

• Antibodies are applied in Western blotting, immunofluorescence, immunohistochemistry, ELISA, IP, and flow cytometry when validated. Conditions (e.g., dilution, incubation time, retrieval) are highly target- and antibody-dependent and must be empirically optimized.

Because application validation for Ab095618 is not listed here, consult the lot-specific datasheet before designing experiments.

Reaction Conditions

Not applicable to small-molecule reaction chemistry. For antibody-based assays, conditions are highly application- and antibody-dependent.

General literature guidance for immunoassays (not item-specific):

• Western blot: Primary antibody incubations commonly range from 1–16 h at 4–25 °C in blocking buffer; optimal dilution must be empirically determined.
• IHC/IF: Incubations typically 1–16 h with appropriate antigen retrieval and blocking; buffer choice (PBS vs TBS) and detergent levels influence background.
• ELISA: Coating, blocking, and detection steps require optimization of antibody concentrations, incubation times, and washing stringency.

For SKU Ab095618, validated applications, recommended dilutions, and incubation parameters are not specified; refer to the CoA/Spec Sheet and perform pilot titrations to define optimal conditions.

Safety and Handling

Hazard classification (item-specific): Not specified for this item; refer to SDS.

• Signal word, H-statements, GHS classification, pictograms: Not specified for this item; refer to SDS.

General laboratory safety for antibody reagents:

• PPE: Wear lab coat, safety glasses, and appropriate gloves. Avoid ingestion, inhalation of aerosols, and skin/eye contact. Use in a clean area to prevent contamination.
• Biological origin: Recombinant production reduces variability and adventitious agents relative to hybridoma-derived material; nonetheless, treat as a laboratory reagent and follow institutional biosafety practices.
• Incompatibilities: Avoid strong oxidants and extremes of pH that can denature proteins. Do not expose to repeated freeze–thaw cycles (Product Data specifies: Avoid repeated freezing and thawing).
• First aid (general): In case of contact, rinse skin/eyes with water. If inhaled, move to fresh air. If ingested, rinse mouth. Seek medical attention as needed. Defer to SDS for authoritative instructions.
• Spill/cleanup: Absorb with disposable towels, clean with aqueous detergent, and dispose according to local regulations for biological/chemical waste.
• Waste: Dispose of protein solutions and contaminated disposables as laboratory chemical/biological waste per institutional guidelines.

Shipping and storage (item-specific): Shipped in ice chest with ice pads; store at −20 °C. Avoid repeated freeze–thaw (both per Product Data).

Solvent Selection

Item-specific formulation details are not specified for SKU Ab095618; refer to CoA/Spec Sheet. General considerations for handling recombinant antibodies:

• Primary medium: Aqueous buffers. Choose isotonic, near-neutral buffers (e.g., PBS, pH ~7.2–7.4; TBS, pH ~7.4–7.6) to maintain conformational integrity.
• Additives: Protein carriers (e.g., 0.1–1% BSA) and nonionic surfactants (e.g., 0.01–0.05% Tween-20) can reduce nonspecific adsorption and improve assay robustness. Glycerol (typically 30–50% v/v) can enable −20 °C storage without freezing, minimizing freeze–thaw.
• Avoid: Organic solvents, high salt/chaotropes, or extreme pH unless intentionally used for elution or regeneration steps; these can denature or aggregate antibodies.
• Dialysis/exchange: If the supplied buffer is incompatible with your assay, perform buffer exchange (spin columns, SEC, or dialysis) under cold conditions to preserve activity.

Comparison (general):

• PBS vs TBS: PBS is widely used in cell-based and immunoassays; TBS may reduce background in certain phospho-protein applications or alkaline phosphatase detection systems where phosphate can interfere.
• With vs without carrier protein: Carrier-free formulations facilitate conjugation (e.g., biotinylation) but may adsorb to plastics; BSA-containing buffers enhance stability for routine immunoassays.

Note: The exact solvent/buffer supplied with Ab095618 is not specified; verify on the datasheet before planning dilutions.

Storage and Reconstitution

Item-specific storage/shipping (from Product Data):

• Storage: Store at −20 °C
• Handling: Avoid repeated freezing and thawing
• Shipping: Ice chest with ice pads

Practical guidance (general; not item-specific specifications):

• Aliquoting: Upon first thaw, subdivide into single-use aliquots in low-bind tubes to prevent freeze–thaw damage. Size aliquots to typical single experiment usage.
• Frozen vs nonfrozen storage: If formulation permits, 30–50% glycerol can enable nonfreezing storage at −20 °C, minimizing ice-crystal damage. Confirm compatibility with your assay and the supplied buffer before adjustment.
• Thawing: Thaw on ice, mix gently by inversion; avoid vortexing. Brief, cold centrifugation can collect condensate.
• Short-term storage: Kept at 2–8 °C for days to weeks depending on preservative presence; this is not specified for Ab095618. Discard if turbidity or precipitate develops.
• Reconstitution: If supplied lyophilized (not specified), reconstitute with sterile, antibody-grade water or buffer to the recommended concentration; allow gentle agitation until fully dissolved, then equilibrate into storage buffer if needed.

Because exact formulation, concentration, and whether the product is liquid or lyophilized are not specified for SKU Ab095618, consult the lot-specific datasheet/CoA for definitive instructions before first use.

Structure and Identity

Item-specific identifiers: Not specified for this item; refer to CoA/Spec Sheet.

• SKU: Ab095618
• Product name: Recombinant Cdc34 Antibody
• Grade/Purity: Recombinant, ExactAb™, Validated (per Product Data)
• Molecular formula: Not applicable for heterogeneous protein preparations; not specified for this item
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet
• CAS, CID, InChIKey, SMILES: Not applicable/not assigned for protein biologics; not specified for this item

Structural description (general, literature):

• Antibodies are Y‑shaped immunoglobulin proteins composed of two heavy and two light chains (∼150 kDa for full-length IgG; format-dependent). Recombinant formats may include full-length IgG, Fab, scFv, or other engineered scaffolds. Specific format for this item is not specified.
• Target: Cdc34 (literature identifiers: also known as UBE2R1) is a ubiquitin-conjugating enzyme (E2) involved in SCF-mediated ubiquitination during cell-cycle control. Epitope, isotype, host species, and clonality are not specified for this item; refer to CoA/Spec Sheet.

2D structure in words (general):

• Antibody domains consist of immunoglobulin beta-sandwich folds with variable regions (VH/VL) forming the antigen-binding site and constant regions conferring effector and stability properties. Exact sequence/structure for this recombinant antibody is not provided here.

Synthetic Utility

Not typically applicable. This product is a recombinant antibody intended for analytical and biochemical applications rather than as a synthetic reagent. It does not participate in named organic transformations or serve as a building block in chemical synthesis.

If conjugation chemistry is planned (general guidance; not item-specific):

• Common linkages: NHS esters for lysine labeling, maleimides for cysteine-specific conjugation, and click-chemistry handles (azide/alkyne) after mild derivatization.
• Considerations: Use carrier-free antibody for controlled degree of labeling, maintain neutral pH for NHS reactions (pH 7.4–8.5), and avoid amine-containing buffers during amine-reactive chemistries. Purify labeled antibody by SEC or desalting to remove unreacted small molecules.

Note: The presence of carrier proteins, amines, or preservatives in Ab095618 (if any) is not specified; consult the datasheet before planning conjugation.

Target Specificity

Not specified for this item; refer to CoA/Spec Sheet.

Only item-specific details such as antigen name/epitope, species reactivity, clone ID, isotype, and cross-reactivity will appear on the product datasheet when available.

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Cdc34 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.