Recombinant CDKN2A/p14ARF Antibody - Anticorpo primario, specific to CDKN2A, Rabbit IgG

Cat. No.: Ab095833

GRADE & PURITY Recombinant, Validated, ExactAb™, See COA

Application

IF/ICC, WB

Host species

Coniglio

Reactive species

Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human CDKN2A/p14ARF Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Relevance
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.

Specifications

Product Name

Recombinant CDKN2A/p14ARF Antibody - Anticorpo primario, specific to CDKN2A, Rabbit IgG

Sinonimi

ARF antibody | CDK4 inhibitor p16 INK4 antibody | CDK4I antibody | CDKN2 antibody | CDKN2A antibody | Cell cycle negative regulator beta antibody | CMM2 antibody | Cyclin dependent kinase 4 inhibitor A antibody | Cyclin dependent kinase inhibitor 2A antib

Specifiche e purezza

Recombinant, ExactAb™, Validated, See COA

Specie ospite

Coniglio

Specificità

CDKN2A

Immunogeno

A synthetic peptide derived from human CDKN2A/p14ARF (AA 75-132).

Controllo positivo

WB: HEK293 and LNCaP cell lysates. IF/ICC: HeLa cells.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant CDKN2A/p14ARF Antibody (Ab095833)

Recombinant CDKN2A/p14ARF Antibody (Ab095833) - Western Blot
All lanes: Recombinant CDKN2A/p14ARF Antibody (Ab095833) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa
Exposure time: 100.0 s

IF/ICC: Recombinant CDKN2A/p14ARF Antibody (Ab095833)

Recombinant CDKN2A/p14ARF Antibody (Ab095833) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant CDKN2A/p14ARF Antibody (Ab095833) at 1/50 dilution.

Applicazione

• Applicazione: Dilution info
• IF/ICC:

  1/50-1/200 ;

• WB:

  1/500-1/1000 ;

Obiettivi associati (umani)

CDKN2A Tbio Tumor suppressor ARF (0 Activities)

Discover Target: CDKN2A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CDKN2A/p14ARF Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.