Recombinant Chk2 Antibody - Anticorpo primario, specific to CHEK2, Rabbit IgG

Cat. No.: Ab096333

GRADE & PURITY Recombinant, ExactAb™, Validated, High Performance, 0.2 mg/mL

Application

IF/ICC, WB

Host species

Coniglio

Reactive species

Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human Chk2 Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/4000
ICC/IF: 1/50-1/200

Specifications

Product Name

Recombinant Chk2 Antibody - Anticorpo primario, specific to CHEK2, Rabbit IgG

Sinonimi

CDS 1 antibody | Cds1 antibody | Cds1 homolog antibody | Checkpoint kinase 2 antibody | Checkpoint like protein CHK2 antibody | CHEK 2 antibody | Chek2 antibody | Chk 2 antibody | CHK2 checkpoint homolog (S. pombe) antibody | CHK2 checkpoint homolog antib

Specifiche e purezza

ExactAb™, Validated, Recombinant, High performance, 0.2 mg/mL

Specie ospite

Coniglio

Specificità

CHEK2

Immunogeno

Recombinant protein of human Chk2 (AA 1-200)

Controllo positivo

WB: K562, HeLa, MCF7, HEK293 and HT-29 cell lysates. ICC/IF: HCT116 cells.

Coniugazione

Unconjugated

Grado

ExactAb™, High Performance, Recombinant, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Antigen affinity purified

Concentrazione

0.2 mg/mL

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant Chk2 Antibody (Ab096333)

Recombinant Chk2 Antibody (Ab096333) - Western Blot
All lanes: Recombinant Chk2 Antibody (Ab096333) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 63 kDa
Exposure time: 6.5 s

Western Blot: Recombinant Chk2 Antibody (Ab096333)

Recombinant Chk2 Antibody (Ab096333) - Western Blot
All lanes: Recombinant Chk2 Antibody (Ab096333) at 1/4000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 37-63 kDa
Exposure time: 6.5 s

IF/ICC: Recombinant Chk2 Antibody (Ab096333)

Recombinant Chk2 Antibody (Ab096333) - IF/ICC
Immunocytochemistry analysis of Chk2 (green) in HCT116 using Recombinant Chk2 Antibody (Ab096333), and cells were counterstained with DAPI (blue).

Applicazione

Applicazione

Dilution info

IF/ICC

1/50-1/200

WB

1/500-1/4000

Obiettivi associati (umani)

CHEK2 Tchem Serine/threonine-protein kinase Chk2 (0 Activities)

Discover Target: CHEK2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CHEK2 Tchem Serine/threonine-protein kinase Chk2 (4015 Activities)

Discover Target: CHEK2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Meccanismi d'azione

Application Protocols

Manufacturer-validated applications for this SKU

• Not specified for this item; refer to CoA/Spec Sheet.

General guidance (not validated for this item)

• If using in WB, IP, IF, IHC, or ELISA, begin with standard starting dilutions (e.g., 1:500–1:2000 for WB, 1–5 µg per IP, 1:100–1:400 for IF/IHC) and optimize empirically. Select positive controls that induce Chk2 activation (e.g., DNA damage treatments) and include negative controls (isotype control, knockdown/knockout lines) to confirm specificity.

Important

• Only the CoA/Spec Sheet should be used for definitive, item-specific protocols and validated applications.

Biological Roles

General biology of the target Chk2 (literature/general knowledge)

• Function: Chk2 (CHEK2) is a serine/threonine checkpoint kinase activated primarily by ATM in response to DNA double-strand breaks. Activation involves phosphorylation (e.g., at Thr68), dimerization, and trans-autophosphorylation.
• Pathways: ATM–Chk2–p53/CDC25 axis orchestrates cell-cycle arrest, DNA repair, and apoptosis under genotoxic stress. Chk2 phosphorylates substrates including CDC25A/C, p53, BRCA1, E2F1, and PML, modulating cell-cycle progression and genome stability.
• Regulation: Activation by DNA damage (ionizing radiation, radiomimetics), deactivation by phosphatases, and modulation via scaffold/adaptor proteins. Subcellular localization can shift upon activation.
• Biomolecular context: Conserved kinase domain with FHA domain mediating phospho-dependent interactions; multiple post-translational modifications influence activity and interactions.

Relevance to antibody use

• Antibodies to Chk2 enable detection/quantification of total or modified (e.g., phospho-Thr68) Chk2 populations, facilitating studies of DNA damage signaling dynamics.

Item-specific note

• This product’s epitope (total vs phospho-specific), species reactivity, and clone/isotype are not specified for this item; consult the CoA/Spec Sheet for definitive details.

Buffer Applications

Applicability

• As an antibody reagent, this product is not used to prepare chemical buffer systems. Instead, it is formulated in or used with biological assay buffers.

General guidance (literature/general knowledge)

• Common working buffers: PBS or TBS at pH ~7.4. For immunoassays, inclusion of 0.05% Tween-20 (PBST/TBST) helps reduce nonspecific binding during wash steps.
• Blocking agents: 3–5% BSA, non-fat dry milk, or serum can block nonspecific sites in WB/IHC/ELISA, selected based on assay and target.
• Additives: Protease and phosphatase inhibitors during lysis preserve antigen integrity; sodium azide can be used as a preservative for storage but is incompatible with live-cell assays and HRP activity at high concentrations.

Item-specific buffer composition

• Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

Context

• Antibodies are aqueous protein reagents; green chemistry considerations focus on buffer composition, preservative choice, cold-chain efficiency, and waste minimization rather than solvent substitution.

Greener practice suggestions (general guidance)

• Buffer systems: Use PBS/TBS without azide where possible to reduce aquatic toxicity; employ sterile filtration and aliquoting to maintain sterility instead of high preservative loads.
• Storage strategy: Aliquot to avoid freeze–thaw cycles and reduce waste from degraded material. Consider glycerol-containing formulations for stable −20°C storage to minimize dry ice shipments.
• Packaging/waste: Opt for recyclable secondary packaging and combine shipments to reduce transport footprint.
• Assay design: Miniaturize assays (e.g., microplate formats, low-volume Westerns) to reduce reagent consumption.

Trade-offs

• Preservative-free buffers are safer for downstream live-cell applications but require stricter aseptic handling and may shorten open-vial stability.
• Glycerol reduces freeze damage but increases solution viscosity and may interfere with certain assays if not diluted appropriately.

Pharmaceutical Uses

Applicability

• This product is for research use only (RUO) and is not intended for diagnostic or therapeutic applications.

General roles of antibodies in pharmaceutical R&D (literature/general knowledge)

• Analytical tools: Antibodies are widely used in QC and development assays (e.g., Western blot, ELISA, immunocapture LC–MS) to characterize targets, pathway modulation, or biomarker changes in preclinical research.
• Process development: Antibodies can be used to track expression/activation of signaling proteins in cell lines used for biologics manufacturing.

Item-specific status

• Pharmacopeial status, excipient role, GMP qualification: Not applicable/not specified for this RUO antibody.

Note

• For any regulated use, obtain appropriate product documentation and consider qualified, GMP-grade reagents instead of RUO materials.

Physical Properties

Item-specific facts (from Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration, formulation buffer, preservatives, carrier proteins: Not specified for this item; refer to CoA/Spec Sheet.

Literature/general values for antibodies (not item-specific)

• Typical IgG molecular mass: ~150 kDa (∼50 kDa heavy chains + ∼25 kDa light chains under reducing SDS-PAGE). Actual mass varies with isotype/glycosylation.
• pI: IgG often pI 6–9 depending on sequence/glycoform; exact pI is sequence-dependent.
• Solubility: Antibodies are soluble in aqueous buffers (e.g., PBS, TBS) with stabilizers (e.g., 0.02–0.05% sodium azide or other preservatives) when applicable; avoid extreme pH and denaturants unless intended for analysis.
• Stability considerations: Sensitive to repeated freeze–thaw; stability enhanced by storage at ≤−20°C or 2–8°C short term, and by inclusion of cryoprotectants (e.g., 10–50% glycerol) when compatible.

Not applicable to proteins

• Boiling/melting point, vapor pressure, logP, refractive index: Not applicable for macromolecular protein reagents.

Important

• Use the product’s CoA/Spec Sheet for exact buffer composition, concentration, and any additives for this specific lot.

Quality and Grades

Item-specific facts (from Product Data)

• Grade/Purity: Recombinant, ExactAb™, Validated, High Performance.

Interpretation (general guidance; not item-specific specifications)

• Recombinant: Produced from a defined sequence in a controlled expression system (e.g., mammalian or other host), supporting enhanced batch-to-batch consistency versus hybridoma or polyclonal sources.
• ExactAb™: Branded designation indicating a defined, sequence-controlled antibody framework for reproducibility and performance (refer to Aladdin Scientific documentation for program-specific criteria).
• Validated: Indicates that the antibody has undergone functional checks; exact assays, species, and validation matrices for this item are not specified—consult the CoA/Spec Sheet.
• High Performance: Marketing/quality descriptor suggesting strong signal-to-noise in intended applications; quantitative performance metrics are not specified for this item.

What to verify on receipt (practical checklist)

• Clone ID or recombinant sequence identifier (if provided), isotype/subclass, host expression system, concentration, and buffer/additives.
• Functional verification data (e.g., WB/IHC/IP/IF), species reactivity, and positive control lysates—Not specified for this item; refer to CoA/Spec Sheet.
• Lot-to-lot correlation protocol and any stability/accelerated aging data if available.

Documentation

• For precise acceptance criteria and analytical tests (purity by SDS-PAGE/SEC-HPLC, endotoxin, bioburden), refer to the product’s CoA/Spec Sheet.

Reaction and Applications

Applicability

• This is an antibody reagent; it is not used as a reactant/solvent in chemical synthesis. The relevant “applications” are immunoassay and protein-detection workflows.

General research applications (literature/general knowledge; not item-specific)

• Western blotting (WB): Detection of Chk2 in cell/tissue lysates under reducing/denaturing conditions; common molecular weight band for full-length Chk2 is ~61–65 kDa, with additional bands possible for phosphorylated/processed forms.
• Immunoprecipitation (IP)/Co-IP: Enrichment of endogenous Chk2 to interrogate DNA damage response complexes.
• Immunofluorescence (IF)/Immunocytochemistry (ICC): Subcellular localization of Chk2 in response to genotoxic stress; fixation/permeabilization conditions influence epitope accessibility.
• ELISA/Target engagement: Quantification of Chk2 levels or phospho-forms when validated pairs are available.

Item-specific application validation

• Manufacturer-validated applications for this SKU: Not specified for this item; refer to CoA/Spec Sheet.

Practical tips

• For phospho-epitopes, ensure appropriate phosphatase inhibition during lysis and processing. Use positive controls (e.g., cells treated with DNA-damaging agents such as doxorubicin or ionizing radiation) and appropriate negative controls (siRNA/CRISPR knockdown) to confirm specificity.

Reaction Conditions

Not chemical reaction conditions; provided as general assay conditions (literature/general knowledge). These are typical starting points and are NOT validated for this specific SKU.

• Western blot (WB):

  • Sample: Denatured/reduced lysates, 20–40 µg total protein per lane.
  • Transfer: PVDF/nitrocellulose membranes.
  • Blocking: 3–5% BSA or milk in TBST for 1 h at RT.
  • Primary incubation: Anti-Chk2 antibody diluted per supplier recommendation (not specified for this item) overnight at 4°C.
  • Secondary: HRP-conjugated anti-species IgG for 1 h at RT; chemiluminescent detection.

• Immunoprecipitation (IP):

  • Lysate: Non-denaturing buffer with protease/phosphatase inhibitors.
  • Antibody: 1–5 µg per IP (optimize); incubate 1–4 h at 4°C with rotation; capture with Protein A/G resin; wash; elute for WB/MS.

• Immunofluorescence (IF/ICC):

  • Fixation: 4% paraformaldehyde (or methanol per epitope), permeabilize with 0.1–0.3% Triton X-100, block with 1–5% BSA/serum.
  • Primary: Anti-Chk2 at optimized dilution; 1–2 h RT or overnight 4°C. Secondary: fluorophore-conjugated anti-species IgG.

Note

• Exact dilutions, incubation times, and positive controls for this SKU are not specified; consult the CoA/Spec Sheet and perform titration/optimization.

Safety and Handling

Item-specific hazard data (from Product Data)

• GHS classification, pictograms, signal word, H-statements: Not specified for this item; refer to SDS.

General laboratory safety guidance (literature/general knowledge)

• Expected hazard profile: Research-grade antibodies are typically classified as non-hazardous mixtures; however, formulations may contain preservatives (e.g., sodium azide) that are acutely toxic and hazardous to aquatic life. Always check the SDS for this SKU.
• PPE: Wear lab coat, safety glasses, and appropriate gloves. Avoid inhalation of aerosols and contact with skin/eyes.
• Handling: Work using good microbiological practices. Avoid repeated freeze–thaw of the vial. Use clean, low-protein-binding tips/tubes to minimize adsorption losses.
• Incompatibilities: Avoid strong oxidizers and proteases. Do not mix with bleach if sodium azide is present (risk of forming explosive metal azides in plumbing).
• First aid (overview; defer to SDS):
  • Skin/eye contact: Rinse with water for several minutes, remove contaminated clothing, seek medical attention if irritation persists.
  • Ingestion/inhalation: Move to fresh air, rinse mouth, seek medical attention as needed.
• Spill response: Absorb with inert material, clean with detergent/water. Dispose according to institutional and local regulations.

Regulatory/use note

• For research use only (RUO). Not for diagnostic, clinical, or therapeutic use.

Solvent Selection

Applicability

• Not a small-molecule reagent; as a protein biologic, this antibody is supplied/reconstituted in aqueous buffer rather than organic solvents.

General guidance (literature/general knowledge)

• Compatible matrices: Phosphate-buffered saline (PBS) or Tris-buffered saline (TBS), typically pH 7.2–7.6. Low levels of detergent (e.g., 0.01% Tween-20) may be used in assay buffers to reduce nonspecific binding where appropriate.
• Stabilizers: Glycerol (10–50%) can be included for freeze protection at −20°C. BSA or carrier proteins may reduce adsorption losses for some applications; confirm compatibility with downstream assays.
• Incompatible media: Strongly denaturing solutions (e.g., 6–8 M urea, >2–4 M guanidinium HCl), extremes of pH (<5 or >9), or organic solvents (e.g., acetonitrile, DMSO, MeOH) are generally unsuitable unless intentionally used for analytical denaturation.

Selection tips

• Choose PBS for most immunoassays and storage; choose TBS for phospho-sensitive systems where phosphate could interfere with certain interactions; add detergents only if validated for your assay system.

Storage and Reconstitution

Item-specific facts (from Product Data)

• Storage conditions: Store at −20°C. Avoid repeated freezing and thawing.
• Shipping: Shipped in ice chest with ice pads.

General best practices (literature/general knowledge)

• Upon receipt: Centrifuge briefly to collect contents. Inspect for any visible precipitate or leakage.
• Aliquoting: Divide into single-use aliquots in low-protein-binding tubes to prevent freeze–thaw damage and adsorption losses.
• Optional cryoprotection: If compatible and if concentrate permits, add sterile glycerol to a final 30–50% v/v for storage at −20°C (do not freeze at −80°C when high glycerol is present).
• Short-term storage: 2–8°C for up to 1–2 weeks may be acceptable if preservative is present; verify stability on the CoA/Spec Sheet.
• Reconstitution: If supplied lyophilized, reconstitute with sterile buffer (e.g., PBS) to the recommended concentration; exact diluent/volume for this item is not specified—refer to CoA/Spec Sheet.
• Freeze–thaw guidance: Thaw on ice, mix gently without vortexing, and keep on ice during use. Avoid multiple freeze–thaw cycles by aliquoting.

Expiration

• Shelf life and open-vial stability: Not specified for this item; refer to CoA/Spec Sheet.

Use restrictions

• For research use only (RUO). Not for diagnostic or therapeutic use.

Structure and Identity

Item-specific facts (from Product Data)

• Product: Recombinant Chk2 Antibody (SKU: Ab096333)
• Grade/Purity: Recombinant, ExactAb™, Validated, High Performance
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• CAS, InChIKey, SMILES: Not applicable/Not specified for antibody reagents.

General description (literature/general knowledge)

• Target: Chk2 (also known as CHEK2), a serine/threonine protein kinase central to the DNA damage response.
• Antibody type: Recombinant antibody (protein biologic; typically an IgG framework unless otherwise stated). Recombinant production supports lot-to-lot consistency versus hybridoma- or polyclonal-derived materials.
• Structural features (antibody, 2D/functional description):
  • Two heavy chains and two light chains arranged in a Y-shaped immunoglobulin architecture with antigen-binding fragments (Fabs) and an effector Fc region (isotype/subclass not specified for this item).
  • Variable domains (VH/VL) determine specificity for epitopes within Chk2; epitope/clone not specified for this item.

Notes

• Stereochemistry/SMILES/InChI conventions do not apply to large protein biologics.
• For clone ID, isotype, and sequence details, consult the CoA/Spec Sheet if provided for this SKU.

Synthetic Utility

Not typically applicable

• As a protein biologic, this antibody is not used as a synthetic building block or reagent in organic synthesis.

Where it can contribute (general laboratory context)

• Analytical enrichment: Immunoprecipitation of Chk2 can enable downstream proteomic analyses (e.g., MS-based phosphoproteomics) but this is an analytical, not synthetic, application.

Item-specific synthetic roles

• None; not applicable.

Target Specificity

Item-specific data (limited to Product Data)

• Target name from product title: Chk2 (CHEK2; checkpoint kinase 2).
• Clone/epitope, isotype/subclass, host species, species reactivity, KD/affinity: Not specified for this item; refer to CoA/Spec Sheet.

General context (not item-specific claims)

• Chk2 is a serine/threonine kinase involved in DNA damage signaling, commonly assessed in human and mammalian models. Antibodies may recognize total or phospho-specific epitopes; the specificity class for this SKU is not specified.