Recombinant DACH1 Antibody - Anticorpo primario, specific to DACH1, Rabbit IgG

Cat. No.: Ab099110

GRADE & PURITY Recombinant, ExactAb™, Validated, High Performance, See COA

Application

WB

Host species

Coniglio

Reactive species

consiglio, Mouse, Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human DACH1 Antibody, Recombinant, could be used for WB and so on.

Specifications

Product Name

Recombinant DACH1 Antibody - Anticorpo primario, specific to DACH1, Rabbit IgG

Sinonimi

DACH antibody | Dach1 antibody | DACH1_HUMAN antibody | Dachshund family transcription factor 1 antibody | dachshund homolog 1 (Drosophila) antibody | Dachshund homolog 1 antibody | Dachsund homolog 1 antibody | FLJ10138 antibody | OTTHUMP00000018494 anti

Specifiche e purezza

Recombinant, ExactAb™, Validated, High Performance, See COA

Meccanismi biochimici e fisiologici

Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the

Specie ospite

Coniglio

Specificità

DACH1

Immunogeno

A synthetic peptide derived from human DACH1 (AA 680-758).

Controllo positivo

WB: HeLa, MCF7, Hep G2, A549, K562, NIH/3T3 and C6 cell lysates.

Coniugazione

Unconjugated

Grado

ExactAb™, High Performance, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Product Line

Prodotti biologici

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant DACH1 Antibody (Ab099110)

Recombinant DACH1 Antibody (Ab099110) - Western Blot
All lanes: Recombinant DACH1 Antibody (Ab099110) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 79 kDa
Observed band size: 83 kDa
Exposure time: 1.4 s

Western Blot: Recombinant DACH1 Antibody (Ab099110)

Recombinant DACH1 Antibody (Ab099110) - Western Blot
All lanes: Recombinant DACH1 Antibody (Ab099110) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 79 kDa
Observed band size: 83 kDa
Exposure time: 4.6 s

Applicazione

Applicazione

Dilution info

WB

1/1000-1/5000

Obiettivi associati (umani)

DACH1 Tbio Dachshund homolog 1 (0 Activities)

Discover Target: DACH1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Item-specific tested applications, recommended dilutions, antigen retrieval conditions, and positive control tissues/cell lines are not specified for this item; refer to the CoA/Spec Sheet.

General, non-item-specific guidance (no dilutions provided)

• Western blot (WB): Use appropriate lysis buffer and protease inhibitors; reduce/denature samples as required; include molecular weight markers; validate specificity with positive/negative controls and knockdown/knockout where feasible.
• Immunocytochemistry/Immunofluorescence (ICC/IF): Fixation method (PFA vs methanol) and permeabilization can affect nuclear protein detection; optimize blocking and wash conditions to minimize background.
• Immunohistochemistry (IHC): Optimize antigen retrieval (heat-induced or enzymatic) and blocking based on tissue; include isotype and no-primary controls.
• Immunoprecipitation (IP): Use low-detergent buffers compatible with nuclear proteins when targeting transcription factors; include DNase treatment as appropriate to disrupt DNA-mediated interactions, if required by the study design.

Always verify the antibody’s format, buffer, and any preservatives on the CoA/Spec Sheet before selecting detection reagents or performing conjugations.

Biological Roles

• Target overview (literature; general background)

  • DACH1 (Dachshund family transcription factor 1) is a mammalian homolog of the Drosophila dachshund gene, functioning predominantly as a transcriptional regulator and co-repressor in developmental pathways. It interfaces with EYA/SIX networks and can modulate cell fate, proliferation, and differentiation programs.
  • Molecular features: contains conserved domains implicated in DNA-binding modulation and protein–protein interactions; participates in chromatin-associated complexes influencing gene expression.

• Cellular and pathway context (literature)

  • Reported roles include regulation of cytoskeletal dynamics, cell cycle checkpoints, and differentiation signals across various tissues during development. In adult tissues, DACH1 expression patterns and regulatory influence are context-dependent and subject to upstream signaling cues.

• Research utility of anti-DACH1 antibodies (general)

  • Antibodies targeting DACH1 are used to study its expression, localization (e.g., nuclear vs cytoplasmic distribution), and interactions in developmental biology and transcriptional regulation research.

Note: Species reactivity, epitope region, and application validation for Ab099110 are not specified for this item; refer to the CoA/Spec Sheet for item-specific details.

Buffer Applications

Not typically applicable as a design topic for this product.

• This is an antibody reagent rather than a buffering compound. Optimal assay buffers depend on application (e.g., WB, IHC, IF, IP), antigen properties, and detection system.
• For this item, specific formulation/buffer details are not specified; consult the CoA/Spec Sheet and SDS. See Application Protocols and Storage & Reconstitution for handling guidance.

Green Alternatives

Not directly applicable.

• Antibody use does not involve selecting between organic solvent systems. Environmental considerations primarily relate to minimizing single-use plastics, selecting lower-impact detergents/buffers where compatible, and responsible cold-chain shipping and energy-efficient storage.
• If conjugation or sample prep uses organic solvents or hazardous reagents, consider greener chemistries and aqueous-compatible labeling kits; ensure compatibility with antibody stability (general guidance).

Pharmaceutical Uses

This product is for research use only.

• No pharmaceutical or clinical use is claimed or supported. It is not intended for diagnostic, therapeutic, or in vivo applications.
• Antibodies can be used as analytical reagents in bioprocess development and QC assays in research settings; any GMP qualification or pharmacopeial status is not specified for this item and should not be assumed.

Physical Properties

• Item-specific physical data

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Concentration/formulation: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • pH, buffer, preservatives: Not specified for this item; refer to CoA/Spec Sheet.

• Not applicable to proteins

  • Boiling/melting point, density, refractive index, logP, SMILES/InChI are not typically defined for macromolecular antibody products.

• General literature values (for context, not item-specific)

  • Typical IgG molecular mass: ~150 kDa (∼50 kDa heavy chain, ∼25 kDa light chain under reducing SDS-PAGE).
  • Isoelectric point (pI): commonly in the pH 6–9 range, isotype- and sequence-dependent.
  • Solubility: antibodies are water-soluble in physiological buffers (e.g., PBS, HEPES) with enhanced stability in the presence of stabilizing proteins/sugars (not specified for this item).

Note: For exact composition (buffer, pH, stabilizers), concentration, and any lyophilized vs liquid format details, consult the product CoA/Spec Sheet for Ab099110.

Quality and Grades

• Item-specific grade statements

  • Grade/Purity: Recombinant, ExactAb™, Validated, High Performance.

• What these mean (general explanations)

  • Recombinant: produced in a defined expression system, delivering lot-to-lot genetic consistency versus hybridoma-derived monoclonals; minimizes variability and eliminates ascites-derived contaminants.
  • ExactAb™ (platform descriptor): typically denotes a recombinant antibody manufactured to a fixed amino-acid sequence with controlled production parameters for high reproducibility. Refer to the CoA for sequence/lot controls specific to Ab099110.
  • Validated: indicates the antibody has undergone internal testing against its intended antigen/assay types. Specific tested applications, species reactivity, and validation datasets are not specified for this item; consult the CoA/Spec Sheet.
  • High Performance: indicates stringent quality expectations on specificity, background, and robustness under recommended conditions; exact acceptance criteria and QC assays (e.g., purity by SDS-PAGE, endotoxin, binding titer, ELISA/Western blot performance) are not specified for this item.

• Practical implications

  • Recombinant format supports reproducible results across lots and facilitates long-term method qualification.
  • Validation scope and any stabilizers/preservatives that affect downstream workflows (e.g., conjugation, live-cell assays) should be verified on the CoA/Spec Sheet before critical experiments.

For certificates, sequence confirmation, and assay validation details specific to Ab099110, refer to the CoA/Spec Sheet.

Reaction and Applications

This section (chemical reaction use as a reagent/solvent) is not applicable to an antibody product.

• Antibodies are used in affinity-based bioassays rather than chemical transformations.
• For practical application domains, see Application Protocols (assay use) and Target Specificity (antigen context). If conjugation chemistry (e.g., labeling) is planned, consult manufacturer guidance for protein conjugation workflows; item-specific conjugation readiness (e.g., carrier-free status) is not specified for this item.

Reaction Conditions

Not applicable in the context of chemical reaction conditions.

• For assay conditions instead of reactions: operating parameters (e.g., incubation times, temperatures, blocking agents, and wash buffers) depend on the specific application (WB, IHC, IF, IP, ELISA). These are not specified for this item; consult the CoA/Spec Sheet for any validated conditions.
• General caution: avoid extremes of pH, high concentrations of denaturants, or organic solvents unless performing analytical gels or antigen retrieval steps that are validated for the assay.

Safety and Handling

• Research use: For research use only.

• GHS classification and hazard statements (item-specific)

  • Signal word: Not specified for this item; refer to SDS.
  • H-statements: Not specified for this item; refer to SDS.
  • Pictograms: Not specified for this item; refer to SDS.

• General handling recommendations (good laboratory practice)

  • PPE: lab coat, gloves, and safety glasses when handling antibody solutions and any associated buffers.
  • Avoid aerosol generation; work on ice for prolonged bench handling to preserve activity.
  • Biological safety: recombinant antibodies are non-infectious proteins; nonetheless, treat as potentially bioactive laboratory reagents and avoid ingestion, inhalation, or skin/eye contact.
  • Incompatibilities: avoid strong oxidizers or extreme pH/denaturing conditions unless intentionally performing SDS-PAGE or similar analyses.
  • Peroxide formation: not applicable (protein solution).

• First-aid overview (general)

  • Skin/eye contact: rinse with water for several minutes; remove contaminated clothing; seek medical advice if irritation persists.
  • Inhalation: move to fresh air; seek medical advice if symptoms occur.
  • Ingestion: rinse mouth; seek medical advice.

• Spills and disposal (general)

  • Absorb small spills with inert material; decontaminate area with appropriate lab disinfectant; dispose according to institutional and local regulations.

Always consult the item’s SDS for authoritative safety and hazard information.

Solvent Selection

This section is not typically applicable to antibody reagents.

• Antibodies are supplied in aqueous buffer systems and are not used as solvents. Selection of organic solvents is generally irrelevant; exposure to strong organic solvents often denatures antibodies.
• For experimental design, focus instead on buffer composition, ionic strength, pH, and additives that preserve antibody conformation and binding (see Storage & Reconstitution).

Storage and Reconstitution

• Item-specific storage and shipping

  • Storage conditions: Store at -20°C. Avoid repeated freezing and thawing.
  • Shipping: Shipped in an ice chest with ice pads (cold chain maintained).

• Handling best practices (general)

  • Upon receipt, inspect for container integrity and keep cold. If recurring use is expected, prepare single-use aliquots in low-protein-binding tubes to avoid freeze–thaw cycles.
  • Thaw on ice; mix gently by inversion—avoid vigorous vortexing or foaming, which can denature antibodies.
  • Short-term bench use should be minimized; keep on ice during setup.

• Reconstitution and buffer details

  • Reconstitution volume, buffer composition, and presence of stabilizers/preservatives are not specified for this item; refer to the CoA/Spec Sheet.
  • If the product is supplied lyophilized (not specified), reconstitution is typically performed with sterile, antibody-compatible buffer (e.g., PBS) to the desired concentration, followed by gentle mixing and brief equilibration on ice. Verify compatibility with downstream assays.

• Long-term storage (general)

  • For multi-month storage, -20°C (as specified) or lower is typical; avoid frost-free freezers. Store protected from light if conjugated labels are present (not specified for this item). Document aliquot dates and freeze–thaw history for QC traceability.

Structure and Identity

This product is a recombinant research-grade antibody directed against the DACH1 protein target.

• Item-specific identifiers

  • SKU: Ab099110
  • Product name: Recombinant DACH1 Antibody
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES / InChI / InChIKey: Not applicable for proteins; not specified for this item.

• Structural features (general, literature)

  • Typical IgG architecture: two heavy chains and two light chains arranged in a Y-shaped quaternary structure stabilized by inter-/intra-chain disulfide bonds.
  • Functional domains: variable regions (VH/VL) forming the antigen-binding fragment (Fab) with complementarity-determining regions (CDRs) that recognize DACH1 epitopes; constant domains (CH/CL) forming the Fc region that mediates effector interactions (isotype-specific; not specified for this item).
  • Post-translational features: recombinant antibodies may be glycosylated in Fc (cell line–dependent), which can influence stability and Fc interactions (item-specific glycosylation not specified).

• 2D description in words (general)

  • Each Fab arm comprises a paired VH–VL β-sandwich framework presenting three CDR loops per chain to form a paratope; the Fc stem comprises CH2–CH3 domains. Hinge-region disulfides connect the two heavy chains, while light chains are linked to their respective heavy chains by disulfides near CH1/CL.

Synthetic Utility

Not applicable.

• Antibodies are biological affinity reagents and are not used as synthetic building blocks or catalysts in organic synthesis.
• If chemical modification is desired (e.g., fluorophore, enzyme, or biotin conjugation), standard protein conjugation chemistries (NHS-ester lysine labeling, maleimide–thiol coupling, click chemistry) can be employed; item-specific suitability (e.g., carrier-free status, presence of amine-containing stabilizers) is not specified for this item and should be verified on the CoA/Spec Sheet before conjugation.

Target Specificity

• Antigen/Target name: DACH1 (Dachshund family transcription factor 1)
• Species reactivity: Not specified for this item; refer to CoA/Spec Sheet.
• Epitope/region: Not specified for this item; refer to CoA/Spec Sheet.
• Clone identifier and isotype: Not specified for this item; refer to CoA/Spec Sheet.
• Host and expression system: Recombinant antibody (exact host/expression system not specified for this item).

Notes

• ExactAb™ recombinant designation indicates sequence-defined production; item-specific sequence/epitope mapping and cross-reactivity profiles should be confirmed on the CoA/Spec Sheet for Ab099110.

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant DACH1 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.