Recombinant DBF4 Antibody - Anticorpo primario, specific to DBF4, Rabbit IgG

Cat. No.: Ab099212

GRADE & PURITY Recombinant, Validated, ExactAb™, See COA

Application

WB

Host species

Coniglio

Reactive species

Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human DBF4 Antibody, Recombinant, could be used for WB and so on.

Specifications

Product Name

Recombinant DBF4 Antibody - Anticorpo primario, specific to DBF4, Rabbit IgG

Sinonimi

Activator of S phase kinase antibody | ASK antibody | CHIF antibody | Chiffon homolog A antibody | DBF4 antibody | DBF4 homolog (S. cerevisiae) antibody | DBF4 homolog antibody | DBF4 type zinc finger containing protein 1 antibody | DBF4 zinc finger A ant

Specifiche e purezza

Recombinant, ExactAb™, Validated, See COA

Meccanismi biochimici e fisiologici

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and '

Specie ospite

Coniglio

Specificità

DBF4

Immunogeno

A synthetic peptide derived from human DBF4 (AA 2-55)

Controllo positivo

WB: Jurkat lysates

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo di catena leggera

kappa

SDS-PAGE

150 kDa

Metodo di purificazione

Protein A purified

Concentrazione

See COA

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant DBF4 Antibody (Ab099212)

Recombinant DBF4 Antibody (Ab099212) - Western Blot
All lanes: Recombinant DBF4 Antibody (Ab099212) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 77 kDa
Observed band size: 73, 75 kDa
The DBF4 protein contains a DNA-binding motif, which affects its migration in Western blot experiments. Its expression pattern aligns with expectations.
Exposure time: 50.0 s

Applicazione

• Applicazione: Dilution info
• WB:

  1/1000-1/2000 ;

Obiettivi associati (umani)

DBF4 Tbio Protein DBF4 homolog A (0 Activities)

Discover Target: DBF4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

DBF4 Tbio Activator of S phase kinase DBF4 (1 Activities)

Discover Target: DBF4

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Meccanismi d'azione

Domande frequenti

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant DBF4 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.