Recombinant E1B-AP5 Antibody - Anticorpo primario, specific to HNRNPUL1, Rabbit IgG

Cat. No.: Ab100771

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, IHC, WB

Host species

Coniglio

Reactive species

Umano

Isotype

Rabbit IgG

Conjugation

Unconjugated

Panoramica

Rabbit anti Human E1B-AP5 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/50-1/200
Protein Function
Acts as a basic transcriptional regulator. Represses basic transcription driven by several virus and cellular promoters. When associated with BRD7, activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation. Plays also a role in mRNA processing and transport. Binds avidly to poly(G) and poly(C) RNA homopolymers in vitro.

Specifications

Product Name

Recombinant E1B-AP5 Antibody - Anticorpo primario, specific to HNRNPUL1, Rabbit IgG

Sinonimi

Adenovirus early region 1B associated protein 5 | Adenovirus early region 1B-associated protein 5 | E130317O14Rik | E1B 55 kDa associated protein 5 | E1B AP5 | E1B-55 kDa-associated protein 5 | E1B-AP5 | E1BAP5 | fb53d10 | fk45c03 | FLJ12944 | Heterogeneo

Specifiche e purezza

ExactAb™, Validated, Recombinant, 0.5 mg/mL

Specie ospite

Coniglio

Specificità

HNRNPUL1

Immunogeno

A synthetic peptide derived from human E1B-AP5 (AA 42-55)

Controllo positivo

WB: A549, A431 and HeLa cell lysates. IHC: Human lung cancer tissue. IF/ICC: Jurkat cells.

Coniugazione

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Metodo di purificazione

Antigen affinity purified

Concentrazione

0.5 mg/mL

Stoccaggio e spedizione

Forma

Liquid

Condizioni di conservazione di stoccaggio

Store at -20°C,Avoid repeated freezing and thawing

Spedito in

Ice chest + Ice pads

Stabilità e conservazione

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Immagini

Western Blot: Recombinant E1B-AP5 Antibody (Ab100771)

Recombinant E1B-AP5 Antibody (Ab100771) - Western Blot
All lanes: Recombinant E1B-AP5 Antibody (Ab100771) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 96 kDa
Observed band size: 117 kDa
Exposure time: 100.0 s

IF: Recombinant E1B-AP5 Antibody (Ab100771)

Recombinant E1B-AP5 Antibody (Ab100771) - IF
Immunocytochemistry analysis of E1B-AP5 (green) in Jurkat using Recombinant E1B-AP5 Antibody (Ab100771),and DAPI(blue).

IHC: Recombinant E1B-AP5 Antibody (Ab100771)

Recombinant E1B-AP5 Antibody (Ab100771) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant E1B-AP5 Antibody (Ab100771). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Applicazione

• Applicazione: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/50 - 1/200;

Obiettivi associati (umani)

HNRNPUL1 Tbio Heterogeneous nuclear ribonucleoprotein U-like protein 1 (0 Activities)

Discover Target: HNRNPUL1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Domande frequenti

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant E1B-AP5 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.